Recombinant mouse MAP3K8/COT protein (His tag) (ab196418)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 38% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant mouse MAP3K8/COT protein (His tag)
See all MAP3K8/COT proteins and peptides -
Biological activity
Specific Activity: 8.5 pmole/min/mg.
Reaction was performed in a kinase buffer (40 mM Tris, 10 mM MgCl2, 0.1 mg/ml BSA) containing 5 µg inactive MEK1, 20 µM ATP, and ab196418 for 60 minutes at 30°C. The amount of ATP transferred was measured.
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Purity
>= 38 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
MENLYASEEPGVYEPSLMTMYPDSNQNEERSESLLRSGQEVPWLSSVRYG TVEDLLAFANHVSNMTKHFYGRRPQECGILLNMVISPQNGRYQIDSDVLL VPWKLTYRNIGSGFVPRGAFGKVYLAQDMKTKKRMACKLIPIDQFKPSDV EIQACFRHENIAELYGAVLWGDTVHLFMEAGEGGSVLEKLESCGPMREFE IIWVTKHILKGLDFLHSKKVIHHDIKPSNIVFMSTKAVLVDFGLSVKMTE DVYLPKDLRGTEIYMSPEVILCRGHSTKADIYSLGATLIHMQTGTPPWVK RYPRSAYPSYLYIIHKQAPPLEDIAGDCSPGMRELIEAALERNPNHRPKA ADLLKHEALNPPREDQPR -
Predicted molecular weight
70 kDa including tags -
Amino acids
30 to 397 -
Tags
His tag N-Terminus , GST tag N-Terminus -
Additional sequence information
NM_007746.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 0.91% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.15% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant mouse MAP3K8/COT protein (His tag) (ab196418)
10% SDS-PAGE analysis of 2.5 μg ab196418 with Coomassie staining.
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Functional Studies - Recombinant mouse MAP3K8/COT protein (His tag) (ab196418)Activity assay of ab196418.
