Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant Mouse IgG2b protein (ab276976)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 92% SDS-PAGE
  • Endotoxin level:
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant Mouse IgG2b protein

  • Purity

    > 92 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    HEK 293 cells

  • Accession

    P01867-2

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Mouse

    • Sequence

      EPSGPISTIN PCPPCKECHK CPAPNLEGGP SVFIFPPNIK DVLMISLTPK VTCVVVDVSE DDPDVQISWF VNNVEVHTAQ TQTHREDYNS TIRVVSTLPI QHQDWMSGKE FKCKVNNKDL PSPIERTISK IKGLVRAPQV YILPPPAEQL SRKDVSLTCL VVGFNPGDIS VEWTSNGHTE ENYKDTAPVL DSDGSYFIYS KLNMKTSKWE KTDSFSCNVR HEGLKNYYLK KTISRSPGL

    • Predicted molecular weight

      26 kDa including tags

    • Molecular weight information

      The recombinant mouse IgG2b-Fc consists of 239 amino acids and has a predicted molecular mass of 26.3 kDa. As a result of glycosylation, the apparent molecular mass of IgG2b-Fc is approximately 37 kDa in SDS-PAGE under reducing conditions.

    • Amino acids

      97 to 335

    • Additional sequence information

      Predicted N-terminus: Glu 97.

    • Specifications

    Our Abpromise guarantee covers the use of ab276976 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituent: 100% PBS

    • Reconstitution
      This information is lot specific. Please contact our technical Support team for details.

    General Info

    • Alternative names

      • gamma2b
      • Ig gamma-2B chain C region
      • Igh-3
      • IGHG2B
      • Immunoglobulin G2b
      see all

    • Relevance

      IgG2 is divided into two subclasses; IgG2a and IgG2b. It is a glycoprotein which consists oftwo identical heavy chains (50 kDa each) and two identical light chains (25 kDa each), to give a combined mass of approximately 150 kDa. The chains are held in place by covalent disulfide bonds. Each light chain contains two immunoglobulin (Ig domains, while the heavy chains contain four Ig domains each. In the middle of each heavy chain is a relative varying portion called the “hinge region” which is unique to each IgG. This region allows for molecular flexibility and sets IgG apart from its IgG counter-parts. Properties of IgG2b include neutralization of toxins and diffusion in the extracellular space.

    • Cellular localization

      Secreted

Images

  • SDS-PAGE - Recombinant Mouse IgG2b protein (ab276976)
    SDS-PAGE - Recombinant Mouse IgG2b protein (ab276976)
    SDS-PAGE analysis of ab276976

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab276976? Please let us know so that we can cite the reference in this datasheet.

ab276976 has not yet been referenced specifically in any publications.

Preparation and Storage

  • gamma2b
  • Ig gamma-2B chain C region
  • Igh-3

Images

  • SDS-PAGE - Recombinant Mouse IgG2b protein (ab276976)
    SDS-PAGE - Recombinant Mouse IgG2b protein (ab276976)
    SDS-PAGE analysis of ab276976

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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