Recombinant Mouse IgG2b protein (ab276976)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 92% SDS-PAGE
- Endotoxin level:
- Suitable for: SDS-PAGE
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Product name
Recombinant Mouse IgG2b protein -
Purity
> 92 % SDS-PAGE. -
Endotoxin level
Expression system
HEK 293 cellsAccession
Protein length
Protein fragmentAnimal free
NoNature
Recombinant-
Species
Mouse -
Sequence
EPSGPISTIN PCPPCKECHK CPAPNLEGGP SVFIFPPNIK DVLMISLTPK VTCVVVDVSE DDPDVQISWF VNNVEVHTAQ TQTHREDYNS TIRVVSTLPI QHQDWMSGKE FKCKVNNKDL PSPIERTISK IKGLVRAPQV YILPPPAEQL SRKDVSLTCL VVGFNPGDIS VEWTSNGHTE ENYKDTAPVL DSDGSYFIYS KLNMKTSKWE KTDSFSCNVR HEGLKNYYLK KTISRSPGL -
Predicted molecular weight
26 kDa including tags -
Molecular weight information
The recombinant mouse IgG2b-Fc consists of 239 amino acids and has a predicted molecular mass of 26.3 kDa. As a result of glycosylation, the apparent molecular mass of IgG2b-Fc is approximately 37 kDa in SDS-PAGE under reducing conditions. -
Amino acids
97 to 335 -
Additional sequence information
Predicted N-terminus: Glu 97.
Specifications
Our Abpromise guarantee covers the use of ab276976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: 100% PBS -
ReconstitutionThis information is lot specific. Please contact our technical Support team for details.
General Info
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Alternative names
- gamma2b
- Ig gamma-2B chain C region
- Igh-3
see all -
Relevance
IgG2 is divided into two subclasses; IgG2a and IgG2b. It is a glycoprotein which consists oftwo identical heavy chains (50 kDa each) and two identical light chains (25 kDa each), to give a combined mass of approximately 150 kDa. The chains are held in place by covalent disulfide bonds. Each light chain contains two immunoglobulin (Ig domains, while the heavy chains contain four Ig domains each. In the middle of each heavy chain is a relative varying portion called the “hinge region” which is unique to each IgG. This region allows for molecular flexibility and sets IgG apart from its IgG counter-parts. Properties of IgG2b include neutralization of toxins and diffusion in the extracellular space. -
Cellular localization
Secreted
Images
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SDS-PAGE - Recombinant Mouse IgG2b protein (ab276976)SDS-PAGE analysis of ab276976
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab276976 has not yet been referenced specifically in any publications.
Preparation and Storage
- gamma2b
- Ig gamma-2B chain C region
- Igh-3
Images
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SDS-PAGE - Recombinant Mouse IgG2b protein (ab276976)SDS-PAGE analysis of ab276976