Recombinant mouse ICAM1 protein (ab277758)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE, MS, HPLC
Preparation and Storage
- Antigen identified by monoclonal antibody BB2
- BB 2
- BB2
Images
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SDS-PAGE - Recombinant mouse ICAM1 protein (ab277758)
SDS-PAGE analysis of ab277758.
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Mass Spectrometry - Recombinant mouse ICAM1 protein (ab277758)Mass determination by ESI-TOF.
Predicted MW is 21191.80 Da (+/- 10 Da by ESI-TOF). Observed MW is 21195.45 Da. Additional masses at 21357.41 and 21519.53 are due to O-linked glycosylation.
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HPLC - Recombinant mouse ICAM1 protein (ab277758)HPLC analysis of ab277758.
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Functional Studies - Recombinant mouse ICAM1 protein (ab277758)Addition of PMA-induced HSB-2 cells to mouse ICAM1 coated plates (12.5 μg/mL, 100 μL/well) led to 40.75% specific cell adherence. Statistical analysis was performed using two-tailed unpaired t-test, **=p