Recombinant mouse Caspase-1 protein (ab52079)
Key features and details
- Expression system: Escherichia coli
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies
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Product name
Recombinant mouse Caspase-1 protein
See all Caspase-1 proteins and peptides -
Biological activity
SPECIFIC ACTIVITY: >3500 units/mg UNIT DEFINITION: One unit of the recombinant caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate Ac-YVAD-pNA (pNA: pnitroanaline) per hour at 37oC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT
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Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
NK EDGTFPGLTG TLKFCPLEKA QKLWKENPSE IYPIMNTTTR TRLALIICNT EFQHLSPRVG AQVDLREMKL LLEDLGYTVK VKENLTALEM VKEVKEFAAC PEHKTSDSTF LVFMSHGIQE GICGTTYSNE VSDILKVDTI FQMMNTLKCP SLKDKPKVII IQACRGEKQG VVLLKDSVRD SEEDFLTDAI FEDDGIKKAH IEKDFIAFCS STPDNVSWRH PVRGSLFIES LIKHMKEYAW SCDLEDIFRK VRFSFEQPEF RLQMPTADRV TLTKRFYLFP GH -
Amino acids
119 to 402 -
Tags
His tag N-Terminus -
Additional sequence information
Large (20 kD) and small (10 kD) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. This protein contains about 284 aa without propeptide.
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Preparation and Storage
- CASP-1
- CASP1
- CASP1 nirs variant 1
