Recombinant human Tryptophan Hydroxylase/TPH protein (Active) (ab206448)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human Tryptophan Hydroxylase/TPH protein (Active) -
Biological activity
Assay Conditions: Reaction was performed at room temperature in assay buffer containing 50 mM MOPS, pH 7.0, 60 μM tryptophan, 100 mM ammonium sulfate, 100 μM ferrous ammonium sulfate, 0.5 mM TCEP, 0.3 mM 6-methyl tetrahydropterin, 0.05 mg/ml catalase, 1 mM DTT and TPH1. Fluorescence signal was measured at Em360nm/Ex300nm.
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Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
VPWFPKKISDLDHCANRVLMYGSELDADHPGFKDNVYRKRRKYFADLAMN YKHGDPIPKVEFTEEEIKTWGTVFQELNKLYPTHACREYLKNLPLLSKYC GYREDNIPQLEDVSNFLKERTGFSIRPVAGYLSPRDFLSGLAFRVFHCTQ YVRHSSDPFYTPEPDTCHELLGHVPLLAEPSFAQFSQEIGLASLGASEEA VQKLATCYFFTVEFGLCKQDGQLRVFGAGLLSSISELKHALSGHAKVKPF DPKITCKQECLITTFQDVYFVSESFEDAKEKMREFTKTIKRPFGVKYNPY TRSIQILKDTKSITSA -
Predicted molecular weight
37 kDa including tags -
Amino acids
105 to 420 -
Tags
His tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.16% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human Tryptophan Hydroxylase/TPH protein (Active) (ab206448)
Activity assay using ab206448
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SDS-PAGE - Recombinant human Tryptophan Hydroxylase/TPH protein (Active) (ab206448)6-20% SDS-PAGE stained with Coomassie Blue.
Lane 1: ab206448 (5 µg)
Lane 2: Protein Marker