Recombinant human RSPO1 protein (ab155744)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: ELISA, SDS-PAGE, Functional Studies
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 0.75% Glycine, 0.61% Tris
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
Images
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SDS-PAGE - Recombinant human RSPO1 protein (ab155744)Human R-Spondin 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Functional Studies - Recombinant human RSPO1 protein (ab155744)Immobilized Human R-Spondin 1, His Tag at 5 µg/mL (100 µL/well) can bind Human SCARB2, Fc Tag with a linear range of 0.02-0.156 µg/mL. -
SDS-PAGE - Recombinant human RSPO1 protein (ab155744)SDS-PAGE analysis of reduced ab155744 stained overnight with Coomassie Blue.
