Recombinant human Raf1 (mutated Y340D + Y341D) protein (Active) (ab271473)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 75% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human Raf1 (mutated Y340D + Y341D) protein (Active)
See all Raf1 proteins and peptides -
Biological activity
Specific activity = 200 pmol/min/µg.
Assay was done in a kinase buffer containing 1 mM DTT using MEK1 (0.1 mg/ml) as a substrate with 5 µM ATP at 30°C for 30 min.
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Purity
>= 75 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
65 kDa including tags -
Amino acids
306 to 648 -
Modifications
mutated Y340D + Y341D -
Tags
GST tag N-Terminus -
Additional sequence information
Genbank: NM_002880
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Preparation and Storage
- c Raf
- C-raf
- C-Raf proto-oncogene, serine/threonine kinase
Images
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Functional Studies - Recombinant Human Raf1 protein (Tagged) (ab271473)
Functional analysis of ab271473.
Specific activity = 200 pmol/min/µg.
Assay was done in a kinase buffer containing 1 mM DTT using MEK1 (0.1 mg/ml) as a substrate with 5 µM ATP at 30°C for 30 min.
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SDS-PAGE - Recombinant Human Raf1 protein (Tagged) (ab271473)SDS-PAGE analysis of ab271473.