Recombinant Human Periostin protein (ab203522)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, WB, ELISA
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
0.05 M Acetate buffer
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ReconstitutionAdd 0.1 M acetate buffer pH 4.0 to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 µg/mL. In higher concentrations the solubility of this antigen is limited. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
Images
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SDS-PAGE - Recombinant Human Periostin protein (ab203522)
12% SDS-PAGE analysis of ab203522.
Lane 1: Reduced and heated sample, 5 μg/lane
Lane 2: Non-reduced and non-heated sample, 5 μg/lane
