Recombinant human PARP15 protein (ab198468)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 55% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human PARP15 protein -
Biological activity
One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
Enzyme reaction is conducted using a PARP15 Assay Kit at room temperature for 30 minutes.
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Purity
>= 55 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSIFYDSMKKRDLSASLNFQS TFSMTTCNLPEHWTDMNHQLFCMVQLEPGQSEYNTIKDKFTRTCSSYAIE KIERIQNAFLWQSYQVKKRQMDIKNDHKNNERLLFHGTDADSVPYVNQHG FNRSCAGKNAVSYGKGTYFAVDASYSAKDTYSKPDSNGRKHMYVVRVLTG VFTKGRAGLVTPPPKNPHNPTDLFDSVTNNTRSPKLFVVFFDNQAYPEYL ITFTA -
Predicted molecular weight
53 kDa including tags -
Amino acids
221 to 444 -
Tags
GST tag N-Terminus -
Additional sequence information
GenBank Accession No. NM_152615
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human PARP15 protein (ab198468)
10% SDS PAGE of 2 μg ab198468 stained with Coomassie.
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Functional Studies - Recombinant human PARP15 protein (ab198468)Specific activity analysis of ab198468.
