Recombinant human Nmnat1/NMNAT protein (ab198473)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human Nmnat1/NMNAT protein
See all Nmnat1/NMNAT proteins and peptides -
Biological activity
Specific Activity: ≥4200 pmol/min/µg
50 μL reaction mixture contained 50 mM Tris-HCl, pH 7.4, 5 mM MgCl2, 0.1 mM NMN, 0.1 mM ATP and various amounts of NMNAT. The reaction was incubated at 37°C for 30 minutes and the amount of ATP consumed was calculated.
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Purity
> 95 % SDS-PAGE.
Lot specific. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MHHHHHHENSEKTEVVLLACGSFNPITNMHLRLFELAKDYMNGTGRYTVV KGIISPVGD AYKKKGLIPAYHRVIMAELATKNSKWVEVDTWESLQKEW KETLKVLRHHQEKLEASDC DHQQNSPTLERPGRKRKWTETQDSSQKKS LEPKTKAVPKVKLLCGADLLESFAVPNL WKSEDITQIVANYGLICVTR AGNDAQKFIYESDVLWKHRSNIHVVNEWIANDISSTKIRRA LRRGQSI RYLVPDLVQEYIEKHNLYSSESEDRNAGVILAPLQRNTAEAKT -
Predicted molecular weight
33 kDa including tags -
Amino acids
2 to 279 -
Tags
His tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.74% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human Nmnat1/NMNAT protein (ab198473)
4-20% SDS PAGE analysis of 2 μg ab198473 stained with Coomassie
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Functional Studies - Recombinant human Nmnat1/NMNAT protein (ab198473)Specific activity of ab198473
