Recombinant human mTOR + MLST8 + Raptor protein (ab196074)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 50% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus, His tag N-Terminus, His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human mTOR + MLST8 + Raptor protein -
Biological activity
Specific Activity: 7.1pmol/min/µg.
Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35 and 2 µM substrate at room temperature for 1 hour.
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Purity
> 50 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
EFMEHSDKGPLPLRDDNGIVLLGERAAKCRAYAKALHYKELEFQKGPTPA ILESLISINNKLQQPEAAAGVLEYAMKHFGELEIQATWYEKLHEWEDALV AYDKKMDTNKDDPELMLGRMRCLEALGEWGQLHQQCCEKWTLVNDETQAK MARMAAAAAWGLGQWDSMEEYTCMIPRDTH DGAFYRAVLALHQDLFSLAQQCIDKARDLLDAELTAMAGESYSRAYGAMV SCHMLSELEEVIQYKLVPERREIIRQIWWERLQGCQRIVEDWQKILMVRS LVVSPHEDMRTWLKYASLCGKSGRLALAHKTLVLLLGVDPSRQLDHPLPT VHPQVTYAYMKNMWKSARKIDAFQHMQHFVQTMQQQAQHAIATEDQQHKQ ELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYSAATEHDRSWYKAWH AWAVMNFEAVLHYKHQNQARDEKKKLRHASGANITNATTAATTAATATTT ASTEGSNSESEAESTENSPTPSPLQKKVTEDLSKTLLMYTVPAVQGFFRS ISLSRGNNLQDTLRVLTLWFDYGHWPDVNEALVEGVKAIQIDTWLQVIPQ LIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVASKSTTTARHNAA NKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEASRLYFGE RNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKS GNVKDLTQAWDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELAVP GTYDPNQPIIRIQSIAPSLQVITSKQRPRKLTLMGSNGHEFVFLLKGHED LRQDERVMQLFGLVNTLLANDPTSLRKNLSIQRYAVIPLSTNSGLIGWVP HCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTLMQKVEVFEHAV NNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRHP SNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTG LDGNYRITCHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKR SRTRTDSYSAGQSVEILDGVELGEPAHKKTGTTVPESIHSFIGDGLVKPE ALNKKAIQIINRVRDKLTGRDFSHDDTLDVPTQVELLIKQATSHENLCQC YIGWCPFW -
Predicted molecular weight
137 kDa including tags -
Amino acids
1362 to 2549 -
Tags
DDDDK tag N-Terminus -
Additional sequence information
NM_004958.
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Amino Acid Sequence 2
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Species
Human -
Sequence
NTSPGTVGSDPVILATAGYDHTVRFWQAHSGICTRTVQHQDSQVNALEVT PDRSMIAAAGYQHIRMYDLNSNNPNPIISYDGVNKNIASVGFHEDGRWMY TGGEDCTARIWDLRSRNLQCQRIFQVNAPINCVCLHPNQAELIVGDQSGA IHIWDLKTDHNEQLIPEPEVSITSAHIDPDASYMAAVNSTGNCYVWNLTG GIGDEVTQLIPKTKIPAHTRYALQCRFSPDSTLLATCSADQTCKIWRTSN FSLMTELSIKSGNPGESSRGWMWGCAFSGDSQYIVTASSDNLARLWCVET GEIKREYGGHQKAVVCLAFNDSVLG -
Predicted molecular weight
37 kDa including tags -
Amino acids
2 to 326 -
Tags
His tag N-Terminus -
Additional sequence information
NM_022372.
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Amino Acid Sequence 3
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Species
Human -
Sequence
ESEMLQSPLLGLGEEDEADLTDWNLPLAFMKKRHCEKIEGSKSLAQSWRM KDRMKTVSVALVLCLNVGVDPPDVVKTTPCARLECWIDPLSMGPQKALET IGANLQKQYENWQPRARYKQSLDPTVDEVKKLCTSLRRNAKEERVLFHYN GHGVPRPTVNGEVWVFNKNYTQYIPLSIYDLQTWMGSPSIFVYDCSNAGL IVKSFKQFALQREQELEVAAINPNHPLAQMPLPPSMKNCIQLAACEATEL LPMIPDLPADLFTSCLTTPIKIALRWFCMQKCVSLVPGVTLDLIEKIPGR LNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNFL LAERIMRSYNCTPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGT AFRHSPFFAEQLTAFQVWLTMGVENRNPPEQLPIVLQVLLSQVHRLRALD LLGRFLDLGPWAVSLALSVGIFPYVLKLLQSSARELRPLLVFIWAKILAV DSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTAFILAVIVNSYHTGQE ACLQGNLIAICLEQLNDPHPLLRQWVAICLGRIWQNFDSARWCGVRDSAH EKLYSLLSDPIPEVRCAAVFALGTFVGNSAERTDHSTTIDHNVAMMLAQL VSDGSPMVRKELVVALSHLVVQYESNFCTVALQFIEEEKNYALPSPATTE GGSLTPVRDSPCTPRLRSVSSYGNIRAVATARSLNKSLQNLSLTEESGGA VAFSPGNLSTSSSASSTLGSPENEEHILSFETIDKMRRASSYSSLNSLIG VSFNSVYTQIWRVLLHLAADPYPEVSDVAMKVLNSIAYKATVNARPQRVL DTSSLTQSAPASPTNKGVHIHQAGGSPPASSTSSSSLTNDVAKQPVSRDL PSGRPGTTGPAGAQYTPHSHQFPRTRKMFDKGPEQTADDADDAAGHKSFI SATVQTGFCDWSARYFAQPVMKIPEEHDLESQIRKEREWRFLRNSRVRRQ AQQVIQKGITRLDDQIFLNRNPGVPSVVKFHPFTPCIAVADKDSICFWDW EKGEKLDYFHNGNPRYTRVTAMEYLNGQDCSLLLTATDDGAIRVWKNFAD LEKNPEMVTAWQGLSDMLPTTRGAGMVVDWEQETGLLMSSGDVRIVRIWD TDREMKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSIRVYDRRMALSE CRVMTYREHTAWVVKASLQKRPDGHIVSVSVNGDVRIFDPRMPESVNVLQ IVKGLTALDIHPQADLIACGSVNQFTAIYNSSGELINNIKYYDGFMGQRV GAISCLAFHPHWPHLAVGSNDYYISVYSVEKRVR -
Predicted molecular weight
150 kDa including tags -
Amino acids
2 to 1335 -
Tags
His tag N-Terminus -
Additional sequence information
NM_020761.
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Preparation and Storage
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1257)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-Raptor antibody (ab189158)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab21536)
- Anti-G protein beta subunit like/GBL antibody (ab25974)
- Anti-mTOR antibody [Y391] (ab32028)
- Anti-Raptor antibody [EP539Y] (ab40768)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Raptor antibody (ab62454)
- Anti-6X His tag® antibody (ab9108)
Images
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SDS-PAGE - Recombinant human mTOR + MLST8 + Raptor protein (ab196074)
10% SDS-PAGE analysis of 3 µg ab196074 with Coomassie staining.
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Functional Studies - Recombinant human mTOR + MLST8 + Raptor protein (ab196074)Activity assay using ab196074.