Recombinant human kynurenine 3-monooxygenase protein (Active) (ab271578)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 60% SDS-PAGE
- Active: Yes
- Tags: His-DDDDK tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
-
Product name
Recombinant human kynurenine 3-monooxygenase protein (Active)
See all kynurenine 3-monooxygenase proteins and peptides -
Biological activity
The specific activity of ab271578 was 100 pmol/min/μg.
ab271578 was tested with various amounts of enzyme. The reactions in KMO Assay Buffer (50 mM Sodium Phosphate, pH 7.5, 0.1% (w/v) Brij-35) contains 200 μM NADPH, 400 μM L-Kynurenine, and various concentrations of KMO. The reaction mixture incubated for 90 minutes while reading the UV absorption signal. For the negative control (blank), assay buffer was added instead of the enzyme. Absorption signals at 340 nm in a plate reader.
-
Purity
>= 60 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MDSSVIQRKK VAVIGGGLVG SLQACFLAKR NFQIDVYEAR EDTRVATFTR GRSINLALSH RGRQALKAVG LEDQIVSQGI PMRARMIHSL SGKKSAIPYG TKSQYILSVS RENLNKDLLT AAEKYPNVKM HFNHRLLKCN PEEGMITVLG SDKVPKDVTC DLIVGCDGAY STVRSHLMKK PRFDYSQQYI PHGYMELTIP PKNGDYAMEP NYLHIWPRNT FMMIALPNMN KSFTCTLFMP FEEFEKLLTS NDVVDFFQKY FPDAIPLIGE KLLVQDFFLL PAQPMISVKC SSFHFKSHCV LLGDAAHAIV PFFGQGMNAG FEDCLVFDEL MDKFSNDLSL CLPVFSRLRI PDDHAISDLS MYNYIEMRAH VNSSWFIFQK NMERFLHAIM PSTFIPLYTM VTFSRIRYHE AVQRWHWQKK VINKGLFFLG SLIAISSTYL LIHYMSPRSF LRLRRPWNWI AHFRNTTCFP AKAVDSLEQI SNLISR -
Predicted molecular weight
58 kDa including tags -
Amino acids
1 to 486 -
Tags
His-DDDDK tag C-Terminus -
Additional sequence information
Genbank: NM_003679
-
Preparation and Storage
-
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 0.01% TCEP, 20% Glycerol (glycerin, glycerine)
100 µg/ml DDDDK peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
-
Functional Studies - Recombinant Human kynurenine 3-monooxygenase protein (Tagged) (ab271578)
Specific activity of ab271578 was 100 pmol/min/μg.
-
SDS-PAGE - Recombinant Human kynurenine 3-monooxygenase protein (Tagged) (ab271578)SDS-PAGE analysis of ab271578.