Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant Human Intrinsic Factor protein (His tag) (ab276557)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant Human Intrinsic Factor protein (His tag)
    See all Intrinsic Factor proteins and peptides

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    HEK 293 cells

  • Accession

    P27352-1

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MAWFALYLLS LLWATAGTST QTQSSCSVPS AQEPLVNGIQ VLMENSVTSS AYPNPSILIA MNLAGAYNLK AQKLLTYQLM SSDNNDLTIG QLGLTIMALT SSCRDPGDKV SILQRQMENW APSSPNAEAS AFYGPSLAIL ALCQKNSEAT LPIAVRFAKT LLANSSPFNV DTGAMATLAL TCMYNKIPVG SEEGYRSLFG QVLKDIVEKI SMKIKDNGII GDIYSTGLAM QALSVTPEPS KKEWNCKKTT DMILNEIKQG KFHNPMSIAQ ILPSLKGKTY LDVPQVTCSP DHEVQPTLPS NPGPGPTSAS NITVIYTINN QLRGVELLFN ETINVSVKSG SVLLVVLEEA QRKNPMFKFE TTMTSWGLVV SSINNIAENV NHKTYWQFLS GVTPLNEGVA DYIPFNHEHI TANFTQY

    • Predicted molecular weight

      45 kDa including tags

    • Molecular weight information

      The recombinant human GIF consists of 410 amino acids and predicts a molecular mass of 44.8 KDa. It migrates as an approximately 49 KDa band in SDS-PAGE under reducing conditions.

    • Amino acids

      1 to 417

    • Tags

      His tag C-Terminus

    • Additional sequence information

      Predicted N-terminus: Ser 19.

    • Specifications

    Our Abpromise guarantee covers the use of ab276557 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.4
      Constituent: 100% PBS

    • Reconstitution
      This information is lot specific. Please contact our technical Support team for details.

    General Info

    • Alternative names

      • Gastric Intrinsic Factor
      • Gastric intrinsic factor (vitamin B synthesis)
      • GIF
      • IF
      • IFMH
      • INF
      • Intrinsic factor
      • TCM3
      • TCN3
      see all

    • Relevance

      Intrinsic factor promotes absorption of the essential vitamin Cobalamin (Cbl) in the ileum by specific receptor mediated endocytosis. Defects in the gene GIF are the cause of hereditary intrinsic factor deficiency (IFD) also called congenital pernicious anemia. IFD is an autosomal recessive disorder characterized by megaloblastic anemia.

    • Cellular localization

      Secreted

Images

  • SDS-PAGE - Recombinant Human Intrinsic Factor protein (ab276557)
    SDS-PAGE - Recombinant Human Intrinsic Factor protein (ab276557)
    SDS-PAGE analysis of ab276557

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab276557? Please let us know so that we can cite the reference in this datasheet.

ab276557 has not yet been referenced specifically in any publications.

Preparation and Storage

  • Gastric Intrinsic Factor
  • Gastric intrinsic factor (vitamin B synthesis)
  • GIF

Images

  • SDS-PAGE - Recombinant Human Intrinsic Factor protein (ab276557)
    SDS-PAGE - Recombinant Human Intrinsic Factor protein (ab276557)
    SDS-PAGE analysis of ab276557

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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