Recombinant human IL-33 protein (ab109065)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
pH: 8.20
Constituents: 0.869% Tris HCl, 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human IL-33 protein (ab109065)Pull down assay:
a) pull down of ab109065 by hST2-Fc
b) pull down of hST2-Fc by ab109065.
5 μg of hST2-Fc (or control Fc protein), 2 μg of ab109065 (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5 ml RIPA buffer overnight at 4°C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of ab109065 or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.
