Recombinant human IL-31 protein (ab106619)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE, HPLC, ELISA, WB
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionThe lyophilised product should be stored at -20oC till use. Once the product has been reconstituted according to the CoA, this can be diluted further in a chosen aqueous buffer containing a carrier protein, such as 0.1% BSA and stored in working aliquots at -20oC for at least 3 months
Images
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Western blot - Recombinant human IL-31 protein (ab106619) Image courtesy of an anonymous Abreview.Whole cell lysate prepared from human eosinophil granulocytes was loaded at 500000 cells.
5-16% gel run under reducing, denaturing conditions.Blocking step performed using 5% BSA for 2 hours at 25°C.
ab106619 used at a 1/200 dilution.Primary antibody used at a 1/100 dilution.
Secondary antibody was an HRP conjugated goat anti-rabbit IgG, used at a 1/200 dilution.The positive control was stimulated with IL-3 (10ng/ml) + IL-5 (10ng/ml) + GM-CSF (10ng/ml) for 30 minutes.
