Recombinant human IKK alpha protein (Active) (ab102103)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, WB, Functional Studies
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Product name
Recombinant human IKK alpha protein (Active) -
Biological activity
Specific activity is 2.5nm/min/mg -
Purity
> 75 % Densitometry.
ab102103 was determined to be >75% pure by densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Molecular weight information
Approx 114 kDa by SDS-PAGE -
Amino acids
1 to 745 -
Tags
GST tag N-Terminus -
Additional sequence information
GenBank: BC092514
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Preparation and Storage
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Applications
SDS-PAGE
Western blot
Functional Studies
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Form
Liquid -
Additional notes
ab204863 (IKB alpha peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Images
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Functional Studies - Recombinant human IKK alpha protein (ab102103)The specific activity of IKK alpha (ab102103) was determined to be 2.9 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human IKK alpha protein (ab102103)SDS PAGE analysis of ab102103 -
SDS-PAGE - Recombinant human IKK alpha protein (ab102103)SDS PAGE analysis of ab102103 -
SDS-PAGE - Recombinant human IKK alpha protein (ab102103)ab102103 was determined to be >75% pure by densitometry. MWt ~114 kDa. -
Functional Studies - Recombinant human IKK alpha protein (ab102103)The specific activity of ab102103 was determined to be 2.5 nmol/mg/min. -
Western blot - Recombinant human IKK alpha protein (ab102103)Anti-IKK alpha antibody [Y463] (ab32041) at 1/10000 dilution +Recombinant human IKK alpha protein (Active) (ab102103) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds