Recombinant human HPRT protein (Active) (ab222951)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: MS, SDS-PAGE, Functional Studies
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Product name
Recombinant human HPRT protein (Active)
See all HPRT proteins and peptides -
Biological activity
Specific activity is > 15 units/mg and is defined as the amount of enzyme that catalyze the formation of 1 µmole of guanosine 5’-monophosphate (GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37°C.
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Purity
> 95 % SDS-PAGE.
ab222951 was purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMATRSPGVVISDDEPGYDLDLFCIPNHYAE DLERVFIPHGLIMDRTERLARDVMKEMGGHHIVALCVLKGGYKFFADLLD YIKALNRNSDRSIPMTVDFIRLKSYCNDQSTGDIKVIGGDDLSTLTGKNV LIVEDIIDTGKTMQTLLSLVRQYNPKMVKVASLLVKRTPRSVGYKPDFVG FEIPDKFVVGYALDYNEYFRDLNHVCVISETGKAKYKA -
Predicted molecular weight
27 kDa including tags -
Amino acids
1 to 218 -
Tags
His tag N-Terminus -
Additional sequence information
NP_000185.
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 20% Glycerol, 0.32% Tris HClThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human HPRT protein (Active) (ab222951)
15% SDS-PAGE analysis of 3 µg ab222951.
