Recombinant Human ECE2 protein (His tag) (ab276228)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 93% SDS-PAGE
- Endotoxin level:
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
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Product name
Recombinant Human ECE2 protein (His tag) -
Purity
> 93 % SDS-PAGE. -
Endotoxin level
Expression system
HEK 293 cellsAccession
Protein length
Protein fragmentAnimal free
NoNature
Recombinant-
Species
Human -
Sequence
GVQYHRDPS HSTCLTEACI RVAGKILESL DRGVSPCEDF YQFSCGGWIR RNPLPDGRSR WNTFNSLWDQ NQAILKHLLE NTTFNSSSEA EQKTQRFYLS CLQVERIEEL GAQPLRDLIE KIGGWNITGP WDQDNFMEVL KAVAGTYRAT PFFTVYISAD SKSSNSNVIQ VDQSGLFLPS RDYYLNRTAN EKVLTAYLDY MEELGMLLGG RPTSTREQMQ QVLELEIQLA NITVPQDQRR DEEKIYHKMS ISELQALAPS MDWLEFLSFL LSPLELSDSE PVVVYGMDYL QQVSELINRT EPSILNNYLI WNLVQKTTSS LDRRFESAQE KLLETLYGTK KSCVPRWQTC ISNTDDALGF ALGSLFVKAT FDRQSKEIAE GMISEIRTAF EEALGQLVWM DEKTRQAAKE KADAIYDMIG FPDFILEPKE LDDVYDGYEI SEDSFFQNML NLYNFSAKVM ADQLRKPPSR DQWSMTPQTV NAYYLPTKNE IVFPAGILQA PFYARNHPKA LNFGGIGVVM GHELTHAFDD QGREYDKEGN LRPWWQNESL AAFRNHTACM EEQYNQYQVN GERLNGRQTL GENIADNGGL KAAYNAYKAW LRKHGEEQQL PAVGLTNHQL FFVGFAQVWC SVRTPESSHE GLVTDPHSPA RFRVLGTLSN SRDFLRHFGC PVGSPMNPGQ LCEVW -
Predicted molecular weight
80 kDa including tags -
Molecular weight information
The recombinant human ECE2 consists of 700 amino acids with the predicted molecular mass of 80.2 kDa. As a result of glycosylation, rhECE2 migrates as an approximately 120-125 kDa band in SDS-PAGE under reducing conditions. -
Amino acids
200 to 883 -
Tags
His tag N-Terminus -
Additional sequence information
Predicted N-terminus: His.
Specifications
Our Abpromise guarantee covers the use of ab276228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: 100% PBS -
ReconstitutionThis information is lot specific. Please contact our technical Support team for details.
General Info
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Relevance
Function: Converts big endothelin-1 to endothelin-1. Also involved in the processing of various neuroendocrine peptides, including neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, and prodynorphin-derived peptides. May limit beta-amyloid peptide accumulation in brain. May also have methyltransferase activity. Tissue specificity: Expressed in brain. Strongly down-regulated in inferior parietal lobe from Alzheimer disease patients (at protein level). Similarity: In the N-terminal section; belongs to the methyltransferase superfamily. In the C-terminal section; belongs to the peptidase M13 family. -
Cellular localization
Golgi apparatus membrane. Cytoplasmic granule membrane.
Images
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SDS-PAGE - Recombinant Human ECE2 protein (ab276228)SDS-PAGE analysis of ab276228
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab276228 has not yet been referenced specifically in any publications.
Preparation and Storage
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SDS-PAGE - Recombinant Human ECE2 protein (ab276228)SDS-PAGE analysis of ab276228
Images
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SDS-PAGE - Recombinant Human ECE2 protein (ab276228)SDS-PAGE analysis of ab276228