Recombinant human Cyclin T1 + Cdk9 protein (Active) (ab271464)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 66% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus, GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human Cyclin T1 + Cdk9 protein (Active) -
Biological activity
26 pmol/min/μg
Assay Conditions: Assay was done in a Kinase buffer using PDKtide (0.1 mg/ml) as a substrate with 10 uM ATP at 30°C for 45 min. The amount of ATP transferred from autophosphorylation as well as substrate phosphorylation was calculated using Kinase-Glo reagent
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Purity
>= 66 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
EGERKNNNKRWYFTREQLENSPSRRFGVDPDKELSYRQQAANLLQDMGQR LNVSQLTINTAIVYMHRFYMIQSFTQFPGNSVAPAALFLAAKVEEQPKKL EHVIKVAHTCLHPQESLPDTRSEAYLQQVQDLVILESIILQTLGFELTID HPHTHVVKCTQLVRASKDLAQTSYFMATNSLHLTTFSLQYTPPVVACVCI HLACKWSNWEIPVSTDGKHWWEYVDATVTLELLDELTHEFLQILEKTPNR LKRIWNWRACEAAKKTKADDRGTDEKTSEQTILNMISQSSSDTTIAGLMS MSTSTTSAVPSLPVSEESSSNLTSVEMLPGKRWLSSQPSFKLEPTQGHRT SENLALTGVDHSLPQDGSNAFISQKQNSKSVPSAKVSLKEYRAKHAEELA AQKRQLENMEANVKSQYAYAAQNLLSHHDSHSSVILKMPIEGSENPERPF LEKADKTALKMRIPVAGGDKAASSKPEEIKMRIKVHAAADKHNSVEDSVT KSREHKEKHKTHPSNHHHHHNHHSHKHSHSQLPVGTGNKRPGDPKHSSQT SNLAHKTYSLSSSFSSSSSTRKRGPSEETGGAVFDHPAKIAKSTKSSSLN FSFPSLPTMGQMPGHSSDTSGLSFSQPSCKTRVPHSKLDKGPTGANGHNT TQTIDYQDTVNMLHSLLSAQGVQPTQPTAFEFVRPYSDYLNPRSGGISSR SGNTDKPRPPPLPSEPPPPLPPLPK -
Actual molecular weight
107 kDa -
Amino acids
2 to 726 -
Tags
GST tag N-Terminus
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Amino Acid Sequence 2
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Species
Human -
Sequence
AKQYDSVECPFCDEVSKYEKLAKIGQGTFGEVFKARHRKTGQKVALKKVL MENEKEGFPITALREIKILQLLKHENVVNLIEICRTKASPYNRCKGSIYL VFDFCEHDLAGLLSNVLVKFTLSEIKRVMQMLLNGLYYIHRNKILHRDMK AANVLITRDGVLKLADFGLARAFSLAKNSQPNRYTNRVVTLWYRPPELLL GERDYGPPIDLWGAGCIMAEMWTRSPIMQGNTEQHQLALISQLCGSITPE VWPNVDNYELYEKLELVKGQKRKVKDRLKAYVRDPYALDLIDKLLVLDPA QRIDSDDALNHDFFWSDPMPSDLKGMLSTHLTSMFEYLAPPRRKGSQITQ QSTNQSRNPATTNQTEFERVF -
Actual molecular weight
69 kDa -
Amino acids
2 to 372 -
Tags
GST tag N-Terminus
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Preparation and Storage
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Cellular localization
Cyclin T1: Nucleus. Cdk9: Nucleus.
Images
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SDS-PAGE - Recombinant Human Cyclin T1 + Cdk9 protein (Tagged) (ab271464)SDS-PAGE analysis of ab271464.
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Functional Studies - Recombinant Human Cyclin T1 + Cdk9 protein (Tagged) (ab271464)Specific activity of ab271464.
Assay Conditions: Assay was done in a Kinase buffer using PDKtide (0.1 mg/ml) as a substrate with 10 uM ATP at 30°C for 45 min. The amount of ATP transferred from autophosphorylation as well as substrate phosphorylation was calculated using Kinase-Glo reagent.