Recombinant human Cyclin T1 + Cdk9 protein (Active) (ab271464)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 66% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus, GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
-
Product name
Recombinant human Cyclin T1 + Cdk9 protein (Active) -
Biological activity
26 pmol/min/μg
Assay Conditions: Assay was done in a Kinase buffer using PDKtide (0.1 mg/ml) as a substrate with 10 uM ATP at 30°C for 45 min. The amount of ATP transferred from autophosphorylation as well as substrate phosphorylation was calculated using Kinase-Glo reagent
-
Purity
>= 66 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Amino Acid Sequence 1
-
Species
Human -
Sequence
EGERKNNNKRWYFTREQLENSPSRRFGVDPDKELSYRQQAANLLQDMGQR LNVSQLTINTAIVYMHRFYMIQSFTQFPGNSVAPAALFLAAKVEEQPKKL EHVIKVAHTCLHPQESLPDTRSEAYLQQVQDLVILESIILQTLGFELTID HPHTHVVKCTQLVRASKDLAQTSYFMATNSLHLTTFSLQYTPPVVACVCI HLACKWSNWEIPVSTDGKHWWEYVDATVTLELLDELTHEFLQILEKTPNR LKRIWNWRACEAAKKTKADDRGTDEKTSEQTILNMISQSSSDTTIAGLMS MSTSTTSAVPSLPVSEESSSNLTSVEMLPGKRWLSSQPSFKLEPTQGHRT SENLALTGVDHSLPQDGSNAFISQKQNSKSVPSAKVSLKEYRAKHAEELA AQKRQLENMEANVKSQYAYAAQNLLSHHDSHSSVILKMPIEGSENPERPF LEKADKTALKMRIPVAGGDKAASSKPEEIKMRIKVHAAADKHNSVEDSVT KSREHKEKHKTHPSNHHHHHNHHSHKHSHSQLPVGTGNKRPGDPKHSSQT SNLAHKTYSLSSSFSSSSSTRKRGPSEETGGAVFDHPAKIAKSTKSSSLN FSFPSLPTMGQMPGHSSDTSGLSFSQPSCKTRVPHSKLDKGPTGANGHNT TQTIDYQDTVNMLHSLLSAQGVQPTQPTAFEFVRPYSDYLNPRSGGISSR SGNTDKPRPPPLPSEPPPPLPPLPK -
Actual molecular weight
107 kDa -
Amino acids
2 to 726 -
Tags
GST tag N-Terminus
-
Amino Acid Sequence 2
-
Species
Human -
Sequence
AKQYDSVECPFCDEVSKYEKLAKIGQGTFGEVFKARHRKTGQKVALKKVL MENEKEGFPITALREIKILQLLKHENVVNLIEICRTKASPYNRCKGSIYL VFDFCEHDLAGLLSNVLVKFTLSEIKRVMQMLLNGLYYIHRNKILHRDMK AANVLITRDGVLKLADFGLARAFSLAKNSQPNRYTNRVVTLWYRPPELLL GERDYGPPIDLWGAGCIMAEMWTRSPIMQGNTEQHQLALISQLCGSITPE VWPNVDNYELYEKLELVKGQKRKVKDRLKAYVRDPYALDLIDKLLVLDPA QRIDSDDALNHDFFWSDPMPSDLKGMLSTHLTSMFEYLAPPRRKGSQITQ QSTNQSRNPATTNQTEFERVF -
Actual molecular weight
69 kDa -
Amino acids
2 to 372 -
Tags
GST tag N-Terminus
-
Preparation and Storage
-
Cellular localization
Cyclin T1: Nucleus. Cdk9: Nucleus.
Images
-
SDS-PAGE - Recombinant Human Cyclin T1 + Cdk9 protein (Tagged) (ab271464)SDS-PAGE analysis of ab271464.
-
Functional Studies - Recombinant Human Cyclin T1 + Cdk9 protein (Tagged) (ab271464)Specific activity of ab271464.
Assay Conditions: Assay was done in a Kinase buffer using PDKtide (0.1 mg/ml) as a substrate with 10 uM ATP at 30°C for 45 min. The amount of ATP transferred from autophosphorylation as well as substrate phosphorylation was calculated using Kinase-Glo reagent.
