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Cancer Cell cycle Kinases/phosphatases Cdks

Recombinant human CDK5 + p25 protein (ab196048)

Recombinant human CDK5 + p25 protein (ab196048)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 90% SDS-PAGE
  • Active: Yes
  • Tags: GST tag N-Terminus, GST tag N-Terminus
  • Suitable for: SDS-PAGE, Functional Studies

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Description

  • Product name

    Recombinant human CDK5 + p25 protein
    See all Cdk5 + Cyclin-dependent kinase 5 activator 1, p25 proteins and peptides
  • Biological activity

    Specific Activity: 312 pmol/min/μg

    Assay was done in a Kinase buffer using Histone H1 as a substrate with 20 μM ATP at 30°C for 30 min. The amount of ATP transferred was calculated using a kinase reagent

  • Purity

    > 90 % SDS-PAGE.

  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

    Q00535
    Q15078
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Amino Acid Sequence 1

    • Species

      Human
    • Sequence

      MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYI DGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGA VLDIRYGVSRIAYSKDFETLK VDFLSKLPEMLKMFKDRLCHKTYLNGD HVTHPDFMLYDALDVVLYMDPMCLDAFPKLV CFKKRIEAIPQIDKYLK SSKYIAWPLQGWQATFGGGDHPPKSDPLVPRGSPGEFMQKY EKLEKIG EGTYGTVFKAKNRETHEIVALKRVRLDDDDEGVPSSALREICLLKELKHK NI VRLHDVLHSDKKLTLVFEFCDQDLKKYFDSCNGDLDPEIVKSFLFQ LLKGLGFCHSRN VLHRDLKPQNLLINRNGELKLADFGLARAFGIPVRC YSAEVVTLWYRPPDVLFGAKLY STSIDMWSAGCIFAELANAGRPLFPG NDVDDQLKRIFRLLGTPTEEQWPSMTKLPDY KPYPMYPATTSLVNVVP KLNATGRDLLQNLLKCNPVQRISAEEALQHPYFSDFCPP
    • Predicted molecular weight

      60 kDa including tags
    • Amino acids

      1 to 292
    • Tags

      GST tag N-Terminus
    • Additional sequence information

      (NM_004935)
    • Amino Acid Sequence 2

    • Species

      Human
    • Sequence

      MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYI DGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGA VLDIRYGVSRIAYSKDFETLK VDFLSKLPEMLKMFKDRLCHKTYLNGD HVTHPDFMLYDALDVVLYMDPMCLDAFPKLV CFKKRIEAIPQIDKYLK SSKYIAWPLQGWQATFGGGDHPPKSDPLVPRGSPGEFAQPP PAQPPAP PASQLSGSQTGGSSSVKKAPHPAVTSAGTPKRVIVQASTSELLRCLGEFL CRRCYRLKHLSPTDPVLWLRSVDRSLLLQGWQDQGFITPANVVFLYML CRDVISSEV GSDHELQAVLLTCLYLSYSYMGNEISYPLKPFLVESCKE AFWDRCLSVINLMSSKMLQ INADPHYFTQVFSDLKNESGQEDKKRLLL GLDR
    • Predicted molecular weight

      50 kDa
    • Amino acids

      99 to 307
    • Tags

      GST tag N-Terminus
    • Additional sequence information

      This product is for the mature full length protein from aa 99 to 307 (Cyclin-dependent kinase 5 activator 1, p25). NM_003885
  • Description

    Recombinant human Cdk5 + Cyclin-dependent kinase 5 activator 1, p25 protein

Preparation and Storage

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Concentration information loading...

Images

  • SDS-PAGE - Recombinant human CDK5 + p25 protein (ab196048)
    SDS-PAGE - Recombinant human CDK5 + p25 protein (ab196048)

    4-20% SDS-PAGE anaysis of 6 μg ab196048 using Coomassie staining

  • Functional Studies - Recombinant human CDK5 + p25 protein (ab196048)
    Functional Studies - Recombinant human CDK5 + p25 protein (ab196048)

    Specific activity of ab196048

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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