Recombinant human CD3 delta + CD3 epsilon (biotinylated ) protein (Active) (Biotin) (ab205994)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus, Fc tag C-Terminus, DDDDK tag C-Terminus, Fc tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Additional notes
The biotin to protein ratio is 2.5-4.5 as determined by the HABA assay.
No activity loss was observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Concentration information loading...
Images
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Functional Studies - Recombinant human CD3 delta + CD3 epsilon (biotinylated ) protein (Biotin) (ab205994)
ab205994 binds OKT3.
Linear range: 2-39 ng/mL.
ab205994 is coated at 1 ug/mL (100 μL/well), on a streptavidin coated plates (QC tested).
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Functional Studies - Recombinant human CD3 delta + CD3 epsilon (biotinylated ) protein (Biotin) (ab205994)Immobilized SP34-2 at 5 μg/mL (100 μL/well) binds ab205994.
Linear range: 1-20 ng/mL (Routinely tested).
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SDS-PAGE - Recombinant human CD3 delta + CD3 epsilon (biotinylated ) protein (Biotin) (ab205994)Reduced ab205994 on SDS-PAGE, stained overnight with Coomassie Blue.
Purity of protein >95%.
The protein migrates as 45-55 kDa under reducing condition due to glycosylation.
