Recombinant human C5 (mutated W917S) protein (Active) (ab256519)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C.
pH: 7.40
Constituents: PBS, 5% Trehalose
0.22 µm filteredThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
Images
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Functional Studies - Recombinant human C5 (mutated W917S) protein (Active) (ab256519)
Immobilized ab256519 at 2 μg/mL (100 μL/well) can bind anti-C5a MAb (Human IgG1) with a linear range of 0.2-6 ng/mL.
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SDS-PAGE - Recombinant human C5 (mutated W917S) protein (Active) (ab256519)Reducing SDS-PAGE - Recombinant human C5 protein (Active) (ab256519).
The gel was stained overnight with Coomassie Blue.
This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.9 kDa (β chain) and 114.3 kDa (α chain). The protein migrates as 80 kDa (β chain) and 110 kDa (α chain) under reducing conditions (SDS-PAGE) due to glycosylation.