Recombinant Human ADAMTS4 protein (ab132081)
Key features and details
- Expression system: Wheat germ
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, WB, ELISA
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Product name
Recombinant Human ADAMTS4 protein -
Expression system
Wheat germ -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MSQTGSHPGRGLAGRWLWGAQPCLLLPIVPLSWLVWLLLLLLASLLPSAR LASPLPREEEIVFPEKLNGSVLPGSGAPARLLCRLQAFGETLLLELEQDS GVQVEGLTVQYLGQAPELLGGAEPGTYLTGTINGDPESVASLHWDGGALL GVLQYRGAELHLQPLEGGTPNSAGGPGAHILRRKSPASGQGPMCNVKAPL GSPSPRPRRAKRFASLSRFVETLVVADDKMAAFHGAGLKRYLLTVMAAAA KAFKHPSIRNPVSLVVTRLVILGSGEEGPQVGPSAAQTLRSFCAWQRGLN TPEDSDPDHFDTAILFTRQVRPQSAPQAMHCTILRSATT -
Predicted molecular weight
62 kDa including tags -
Amino acids
1 to 339 -
Tags
GST tag N-Terminus
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Preparation and Storage
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Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 4
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4
- A disintegrin like and metalloprotease with thrombospondin type 1 motif 4
see all -
Function
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-
-Ala-393' site. -
Tissue specificity
Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 1 TSP type-1 domain. -
Domain
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human ADAMTS4 protein (ab132081)12.5% SDS-PAGE analysis of ab132081 stained with Coomassie Blue.