Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (ab230255)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, WB, ELISA
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Product name
Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Predicted molecular weight
50 kDa including tags -
Tags
His tag C-Terminus
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: PBS
(supplemented with glycerol, arginine and glutamic acid).
Images
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SDS-PAGE - Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (ab230255)
SDS-PAGE analysis of 1 µg and 5 µg (lanes 1-2) of ab230255 under denaturing and reducing conditions.
Two prominent bands: E3E2 at ~50 kDa and cleaved E2 at ~40 kDa.
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Western blot - Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (ab230255)All lanes : anti-EEEV E2 rabbit polyclonal antibody at 0.5 µg/ml
Lane 1 :Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (ab230255) at 0.05 µg
Lane 2 :Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (ab230255) at 0.1 µg
Lane 3 :Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (ab230255) at 0.2 µg
Secondary
All lanes : anti-rabbit IgG HRP conjugateFollowed by TMB substrate.
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ELISA - Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (ab230255)Plate was coated with ab230255 starting at 800 ng/well, serially diluted in DPBS. Washed plate was detected using Rabbit Anti-EEEV E2 pAb at 1 µg/mL. OD650 is reported.