Recombinant Cytomegalovirus Glycoprotein B (Fc Chimera) (ab206020)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 85% SDS-PAGE
- Endotoxin level:
- Suitable for: SDS-PAGE
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Product name
Recombinant Cytomegalovirus Glycoprotein B (Fc Chimera)
See all Cytomegalovirus Glycoprotein B proteins and peptides -
Purity
> 85 % SDS-PAGE. -
Endotoxin level
Expression system
HEK 293 cellsAccession
Protein length
Protein fragmentAnimal free
NoNature
Recombinant-
Species
Cytomegalovirus -
Sequence
VSSSSTRGTSATHSHHSSHTTSAAHSRSGSVSQRVTSSQTVSHGVNETIY NTTLKYGDVVGVNTTKYPYRVCSMAQGTDLIRFERNIVCTSMKPINEDLD EGIMVVYKRNIVAHTFKVRVYQKVLTFRRSYAYIHTTYLLGSNTEYVAPP MWEIHHINSHSQCYSSYSRVIAGTVFVAYHRDSYENKTMQLMPDDYSNTH STRYVTVKDQWHSRGSTWLYRETCNLNCMVTITTARSKYPYHFFATSTGD VVDISPFYNGTNRNASYFGENADKFFIFPNYTIVSDFGRPNSALETHRLV AFLERADSVISWDIQDEKNVTCQLTFWEASERTIRSEAEDSYHFSSAKMT ATFLSKKQEVNMSDSALDCVRDEAINKLQQIFNTSYNQTYEKYGNVSVFE TTGGLVVFWQGIKQKSLVELERLANRSSLNLTHNRTKRSTDGNNATHLSN MESVHNLVYAQLQFTYDTLRGYINRALAQIAEAWCVDQRRTLEVFKELSK INPSAILSAIYNKPIAARFMGDVLGLASCVTINQTSVKVLRDMNVKESPG RCYSRPVVIFNFANSSYVQYGQLGEDNEILLGNHRTEECQLPSLKIFIAG NSAYEYVDYLFKRMIDLSSISTVDSMIALDIDPLENTDFRVLELYSQKEL RSSNVFDLEE IMREFNSYKQRVKYVEDK -
Predicted molecular weight
104 kDa including tags -
Amino acids
23 to 700 -
Additional sequence information
Fc Chimera is fused with a Human IgG1 Fc tag at the C-terminus. The furin cleavage site is mutated from 'RTKR' to 'TTQT'.
Specifications
Our Abpromise guarantee covers the use of ab206020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 0.61% Tris, 0.75% Glycine, L-Arginine, Sodium chloride, 5% Trehalose -
ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
General Info
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Alternative names
- CMV glycoprotein B
- Glycoprotein B
- HCMV
see all -
Relevance
Human cytomegalovirus (HCMV) glycoprotein B (gB) is an abundant virion envelope protein that has been shown to be essential for the infectivity of HCMV. HCMV gB is also one of the most immunogenic virus-encoded proteins, and a significant fraction of virus neutralizing antibodies are directed at gB. -
Cellular localization
Virus envelope.
Images
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SDS-PAGE - Recombinant Cytomegalovirus Glycoprotein B (Fc Chimera) (ab206020)
SDS-PAGE analysis of reduced ab206020, staining overnight with Coomassie Blue. DTT-reduced Protein migrates as 140-150 kDa in SDS-PAGE due to glycosylation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab206020 has not yet been referenced specifically in any publications.
Preparation and Storage
- CMV glycoprotein B
- Glycoprotein B
- HCMV
Images
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SDS-PAGE - Recombinant Cytomegalovirus Glycoprotein B (Fc Chimera) (ab206020)
SDS-PAGE analysis of reduced ab206020, staining overnight with Coomassie Blue. DTT-reduced Protein migrates as 140-150 kDa in SDS-PAGE due to glycosylation.