Rat MMP8 ELISA Kit (ab100779)
Key features and details
- Sensitivity: 50 pg/ml
- Range: 0.05 ng/ml - 40 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 160X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-rat MMP8 2 vials MMP8 Microplate (12 x 8 wells) 1 unit Recombinant rat MMP8 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Can degrade fibrillar type I, II, and III collagens. -
Tissue specificity
Neutrophils. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Cytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules. - Information by UniProt
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Alternative names
- CLG 1
- CLG1
- Collagenase 1
see all -
Database links
- Entrez Gene: 63849 Rat
- SwissProt: O88766 Rat
- Unigene: 44474 Rat
Images
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Typical data B
Representative standard curve using ab100779
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Typical data ARepresentative standard curve using ab100779
