Rat CXCL3 ELISA Kit (ab243683)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 16 pg/ml
- Range: 63 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat CXCL3 ELISA Kit
See all GRO gamma kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% supernatant 8 3.1% Inter-assay Sample n Mean SD CV% supernatant 3 10.2% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
16 pg/ml -
Range
63 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 103 88% - 97% Serum 103 95% - 111% Hep Plasma 96 94% - 97% EDTA Plasma 88 82% - 93% Cit plasma 96 94% - 97% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat CXCL3 ELISA Kit (ab243683) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL3 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat CXCL3 with 16 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat CXCL3 Capture Antibody 1 x 600µl 10X Rat CXCL3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Plate Seals 1 unit Rat CXCL3 Lyophilized Recombinant Protein 2 vials Sample Diluent 50BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsN-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-X-C motif chemokine 3
- C-X-C motif chemokine ligand 3
- Chemokine (C X C motif) ligand 3
see all -
Database links
- Entrez Gene: 171551 Rat
- SwissProt: Q10746 Rat
- Unigene: 10525 Rat
Images
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Other - Rat CXCL3 ELISA Kit (ab243683)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Rat CXCL3 Standard Curve Comparison.Standard Curve comparison between rat CXCL3 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of rat CXCL3 standard curve in Sample Diluent 50BS.CXCL3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of rat CXCL3 standard curve in Sample Diluent NS.The CXCL3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CXCL3 in spleen supernatant and lung supernatant.The concentrations of CXCL3 were measured in duplicates, interpolated from the CXCL3 standard curves and corrected for sample dilution. Undiluted samples are as follows: 1:400 spleen supernatant and 1:800 lung supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL3 concentration was determined to be 485 ng/mL in spleen supernatant and 1,677 ng/mL lung supernatant.
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Sandwich ELISA - Rat CXCL3 ELISA Kit (ab243683)To learn more about the advantages of recombinant antibodies see here.
