Rat CRP ELISA Kit (C-Reactive Protein) (ab256398)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.9 pg/ml
- Range: 19.5 pg/ml - 1250 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat CRP ELISA Kit (C-Reactive Protein)
See all C Reactive Protein kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 4.2% Inter-assay Sample n Mean SD CV% Extract 3 4.4% -
Sample type
Cell culture supernatant, Urine, Serum, Tissue Extracts, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.9 pg/ml -
Range
19.5 pg/ml - 1250 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 99 97% - 101% Urine 94 90% - 98% Serum 103 103% - 104% Tissue Extracts 105 101% - 109% Cell culture media 113 112% - 115% Hep Plasma 117 116% - 119% EDTA Plasma 111 103% - 118% Cit plasma 114 109% - 124% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat
Does not react with: Cow -
Product overview
Rat CRP ELISA Kit (C-Reactive Protein) (ab256398) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CRP (C-Reactive Protein) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, urine, tissue extracts, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat CRP (C-Reactive Protein) with 3.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CRP (C-Reactive Protein) displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. CRP can interact with DNA and histones and it may scavenge nuclear material released from damaged circulations cells. CRP is secreted; it forms a homopentamer pentaxin (or pentraxin) which have a discoid arrangement of 5 non-covalently bound subunits. CRP binds 2 calcium ions per subunit. The concentration of CRP in plasma increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL1/interleukin-1 and IL6/interleukin-6.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat CRP (C-Reactive protein) Capture Antibody 1 x 600µl 10X Rat CRP (C-Reactive protein) Detector Antibody 2 vials 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Plate Seals 1 unit Rat CRP (C-Reactive protein) Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. -
Tissue specificity
Found in plasma. -
Sequence similarities
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Pentraxin 1, short
- C reactive protein
- C reactive protein pentraxin related
see all -
Database links
- Entrez Gene: 25419 Rat
- SwissProt: P48199 Rat
- Unigene: 16463 Rat
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of rat CRP standard curve in Sample Diluent NS.The CRP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CRP in rat serum and plasma samples.The concentrations of CRP were measured in duplicates, interpolated from the CRP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:400,000, plasma (citrate) 1:200,000, plasma (heparin) 1:400,000, and plasma (EDTA) 1:200,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 326.3 µg/mL in neat serum, 170.1 µg/mL in neat plasma (citrate), 307.1 µg/mL in neat plasma (heparin), and 231.1 µg/mL in neat plasma (EDTA).
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Interpolated concentrations of native CRP in rat urine and tissue culture supernatant samples.The concentrations of CRP were measured in duplicates, interpolated from the CRP standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 1:50, rat heart tissue supernatant 1:200, and rat spleen tissue supernatant 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 36.29 ng/mL in neat urine, 105.0 ng/mL in neat rat heart tissue supernatant, and 87.24 ng/mL in neat rat spleen tissue supernatant.
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Interpolated concentrations of native CRP in rat heart tissue extract based on a 1 µg/mL extract load.The concentrations of CRP were measured in duplicate and interpolated from the CRP standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 359.0 pg/mL in rat heart tissue extract.
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Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (ab256398)To learn more about the advantages of recombinant antibodies see here.
