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Rat CRP ELISA Kit (C-Reactive Protein) (ab256398)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 3.9 pg/ml
  • Range: 19.5 pg/ml - 1250 pg/ml
  • Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Rat

Overview

  • Product name

    Rat CRP ELISA Kit (C-Reactive Protein)
    See all C Reactive Protein kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Extract 8 4.2%
    Inter-assay
    Sample n Mean SD CV%
    Extract 3 4.4%

  • Sample type

    Cell culture supernatant, Urine, Serum, Tissue Extracts, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    3.9 pg/ml

  • Range

    19.5 pg/ml - 1250 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 99 97% - 101%
    Urine 94 90% - 98%
    Serum 103 103% - 104%
    Tissue Extracts 105 101% - 109%
    Cell culture media 113 112% - 115%
    Hep Plasma 117 116% - 119%
    EDTA Plasma 111 103% - 118%
    Cit plasma 114 109% - 124%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Rat
    Does not react with: Cow

  • Product overview

    Rat CRP ELISA Kit (C-Reactive Protein) (ab256398) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CRP (C-Reactive Protein) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, urine, tissue extracts, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat CRP (C-Reactive Protein) with 3.9 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    CRP (C-Reactive Protein) displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. CRP can interact with DNA and histones and it may scavenge nuclear material released from damaged circulations cells. CRP is secreted; it forms a homopentamer pentaxin (or pentraxin) which have a discoid arrangement of 5 non-covalently bound subunits. CRP binds 2 calcium ions per subunit. The concentration of CRP in plasma increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL1/interleukin-1 and IL6/interleukin-6.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Example of rat CRP standard curve in Sample Diluent NS.
    Example of rat CRP standard curve in Sample Diluent NS.

    The CRP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native CRP in rat serum and plasma samples.
    Interpolated concentrations of native CRP in rat serum and plasma samples.

    The concentrations of CRP were measured in duplicates, interpolated from the CRP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:400,000, plasma (citrate) 1:200,000, plasma (heparin) 1:400,000, and plasma (EDTA) 1:200,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 326.3 µg/mL in neat serum, 170.1 µg/mL in neat plasma (citrate), 307.1 µg/mL in neat plasma (heparin), and 231.1 µg/mL in neat plasma (EDTA).

  • Interpolated concentrations of native CRP in rat urine and tissue culture supernatant samples.
    Interpolated concentrations of native CRP in rat urine and tissue culture supernatant samples.

    The concentrations of CRP were measured in duplicates, interpolated from the CRP standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 1:50, rat heart tissue supernatant 1:200, and rat spleen tissue supernatant 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 36.29 ng/mL in neat urine, 105.0 ng/mL in neat rat heart tissue supernatant, and 87.24 ng/mL in neat rat spleen tissue supernatant.

  • Interpolated concentrations of native CRP in rat heart tissue extract based on a 1 µg/mL extract load.
    Interpolated concentrations of native CRP in rat heart tissue extract based on a 1 µg/mL extract load.

    The concentrations of CRP were measured in duplicate and interpolated from the CRP standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 359.0 pg/mL in rat heart tissue extract.

  • Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (ab256398)
    Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (ab256398)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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