Rat CCL4 ELISA Kit (ab240680)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 6.9 pg/ml
- Range: 11.7 pg/ml - 750 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat CCL4 ELISA Kit
See all CCL4/MIP-1 beta kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% spleen 8 5.8% Inter-assay Sample n Mean SD CV% spleen 3 7.1% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
6.9 pg/ml -
Range
11.7 pg/ml - 750 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 98 95% - 100% Serum 90 83% - 94% Hep Plasma 94 90% - 98% EDTA Plasma 96 88% - 101% Cit plasma 99 92% - 106% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat CCL4 ELISA Kit (ab240680) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL4 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat CCL4 with 6.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat CCL4 Capture Antibody 1 x 600µl 10X Rat CCL4 Detector Antibody 2 vials 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Plate Seals 1 unit Rat CCL4 Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsN-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- MIP 1 beta
- Secreted protein G 26
- ACT 2
see all -
Database links
- Entrez Gene: 116637 Rat
- SwissProt: P50230 Rat
- Unigene: 37880 Rat
Images
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Other - Rat CCL4 ELISA Kit (ab240680)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of rat CCL4 standard curve in Sample Diluent NS.The CCL4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of spiked CCL4 in rat serum and plasma samples.The concentrations of CCL4 were measured in duplicates, interpolated from the CCL4 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 50%, plasma (heparin) 50% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). -
Interpolated concentrations of CCL4 in rat stimulated PBMC and spleen samples.Rat PBMCS were cultured in RPMI supplemented with 10% fetal calf serum. Cells were cultured for days in the presence or absence of PHA. Rat Spleen was cultured in the presence or absence of PHA and rat IL2 for 6 days. The concentrations of CCL4 were measured in duplicate and interpolated from the CCL4 standard curve and corrected for sample dilution. Undiluted samples are as follows: 3% PBMC culture supernatant and 25% spleen culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL4 concentration was determined to be 5,330 pg/mL in stimulated PBMC, 720 pg/mL in unstimulated PBMC, and 2,051 pg/mL in stimulated spleen. -
Sandwich ELISA - Rat CCL4 ELISA Kit (ab240680)To learn more about the advantages of recombinant antibodies see here.
