Ran Activation Assay Kit (ab211172)
Key features and details
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Ran Activation Assay Kit -
Sample type
Tissue, Adherent cells, Suspension cells -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Dog, Human -
Product overview
Ran Activation Assay Kit (ab211172) utilizes RanBP1 Agarose beads to selectively isolate and pull-down the active form of Ran from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Ran is detected by western blot analysis using an anti-Ran antibody, which reacts with the human, mouse, rat, dog and chicken protein.
Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.
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Notes
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.
Ran (Ras-related nuclear protein), a 26 kDa protein of the Ras superfamily, is involved in a variety of biological response pathways that include chromatin condensation, cell cycle progression, and translocation of RNA/proteins through the nuclear pore complex. Ran acts a negative regulator of the kinase activity of CRK1 and VRK2. Like other small GTPases, Ran regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Ran binds specifically to RanBP1 to control downstream signaling cascades.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 20 tests 100X GDP 1 x 50µl 100X GTPyS 1 x 50µl 5X Assay/Lysis Buffer 1 x 30ml ab211182 - RanBP1 Agarose Beads 1 x 800µl Anti-Ran Mouse monoclonal antibody 1 x 40µl GTPase immunoblot Positive Control 1 x 100µl -
Research areas
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Function
GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.
Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases. -
Tissue specificity
Expressed in a variety of tissues. -
Sequence similarities
Belongs to the small GTPase superfamily. Ran family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Nucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Alternative names
- Androgen receptor associated protein 24
- Androgen receptor-associated protein 24
- ARA 24
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Database links
- Entrez Gene: 396193 Chicken
- Entrez Gene: 442976 Dog
- Entrez Gene: 5901 Human
- Entrez Gene: 100045999 Mouse
- Entrez Gene: 19384 Mouse
- Entrez Gene: 84509 Rat
- Omim: 601179 Human
- SwissProt: P42558 Chicken
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Images
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Ran Activation Assay Kit (ab211172)
Ran positive control (10 µL of 293 cell lysate at 0.5 mg/mL) detected by immunoblot.
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Ran Activation Assay Kit (ab211172)Ran activation assay. Lane 1. NIH 3T3 cell lysate loaded with GDP and incubated with RanBP1 Agarose beads. Lane 2. NIH 3T3 cell lysate loaded with GTPγS and incubated with RanBP1 Agarose beads.