Rabbit NCAM1 ELISA Kit (CD56) (ab273250)
Key features and details
- Sensitivity: 0.45 ng/ml
- Range: 0.41 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rabbit
Overview
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Product name
Rabbit NCAM1 ELISA Kit (CD56)
See all NCAM1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.45 ng/ml -
Range
0.41 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 140.8 136% - 145% Plasma 140.4 134% - 147% Cell culture media 105.9 87% - 120% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rabbit -
Product overview
Rabbit NCAM1 ELISA Kit (CD56) (ab273250) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of rabbit NCAM1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for rabbit NCAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and NCAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-rabbit NCAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NCAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Anti-Rabbit NCAM1 (CD56) coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Rabbit NCAM1 (CD56) Antibody 2 vials Rabbit NCAM1 (CD56) Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. -
Sequence similarities
Contains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- antigen MSK39 identified by monoclonal antibody 5.1H11
- antigen recognized by monoclonal antibody 5.1H11
- CD56
see all
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.