Rabbit Anti-Human IgA H&L (HRP) (ab8510)
Key features and details
- Rabbit Anti-Human IgA H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Suitable for: Dot blot, ELISA
Overview
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Product name
Rabbit Anti-Human IgA H&L (HRP)
See all IgA secondary antibodies -
Host species
Rabbit -
Target species
Human -
Specificity
Antibodies were raised in rabbit to human IgA -
Tested applications
Suitable for: Dot blot, ELISAmore details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
pH: 7.50
Constituents: 0.164% Sodium phosphate, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgM and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgA. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgA a minimum of 65% protein bound. -
Conjugation notes
The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate. -
Clonality
Polyclonal -
Research areas