Proteasome Activity Assay Kit (ab107921)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell Lysate
Overview
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Product name
Proteasome Activity Assay Kit -
Detection method
Fluorescent -
Sample type
Cell Lysate -
Assay type
Enzyme activity -
Assay time
1h 00m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Proteasome Activity Assay Kit (ab107921) takes advantage of the chymotrypsin-like activity, using an AMC-tagged peptide substrate (Proteasome Substrate (Succ-LLVY-AMC in DMSO), which releases free, highly fluorescent AMC (Ex/Em 350/440 nm) in the presence of proteolytic activity.
The kit also includes a positive control (Jurkat Cell lysate with significant proteasome activity) and a specific proteasome inhibitor MG-132 which suppresses all proteolytic activity due to proteasomes. This permits differentiation of proteasome activity from other protease activity which may be present in samples.
Proteasome activity assay protocol summary:
- add samples, standards and positive control to wells
- add proteasome inhibitor to half of sample and positive control wells
- add proteasome substrate to sample and control wells
- incubate for 20 min
- analyze with microplate reader, incubate for further 30 min, analyze again -
Notes
The 20S proteasome assembly is the functional protease structure with chymotrypsin-like, trypsin-like and caspase-like protease activities
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Storage buffer
Preservative: None
Constituents: DMSO -
Components Identifier 100 tests AMC Standard (1mM in DMSO) Yellow 1 x 100µl Positive Control (lyophilized) Green 1 vial Proteasome Substrate (in DMSO) Red 1 x 100µl Proteasome Assay Buffer WM 1 x 25ml Proteasome Inhibitor (in DMSO) Blue 1 x 100µl -
Research areas
Images
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Functional Studies - Proteasome Activity Assay Kit (ab107921)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Functional Studies - Proteasome Activity Assay Kit (ab107921)Proteasome Activity measured in Jurkat lysate
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Functional Studies - Proteasome Activity Assay Kit (ab107921)Proteasome Activity measured in HeLa lysate
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Functional Studies - Proteasome Activity Assay Kit (ab107921)Proteasome Activity measured in cell lysates showing activity (mU) per 1 mln cells.
Samples with the concentration of 3.3e6 cells/mL were used. 50 μl of each sample was used.