Porcine TIMP1 ELISA Kit (ab273223)
Key features and details
- Sensitivity: 18 pg/ml
- Range: 16.38 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human, Pig
Overview
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Product name
Porcine TIMP1 ELISA Kit
See all TIMP1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
18 pg/ml -
Range
16.38 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 131.5 107% - 141% Serum 75.84 70% - 83% Plasma 93.73 77% - 109% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human, Pig -
Product overview
Porcine TIMP1 ELISA Kit (ab273223) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine TIMP1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for porcine TIMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-porcine TIMP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Porcine TIMP1 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Porcine TIMP1 Antibody 2 vials Porcine TIMP1 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. -
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
Post-translational
modificationsThe activity of TIMP1 is dependent on the presence of disulfide bonds. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Clgi
- Collagenase inhibitor
- Collagenase inhibitor, Human
see all -
Database links
- Entrez Gene: 7076 Human
- Entrez Gene: 396862 Pig
- Omim: 305370 Human
- SwissProt: P01033 Human
- SwissProt: P35624 Pig
- Unigene: 522632 Human
Images
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Porcine TIMP1 Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Porcine TIMP1 ELISA kit (ab273223) Standard curve.