Porcine TGF alpha ELISA Kit (ab273225)
Key features and details
- Sensitivity: 0.6 pg/ml
- Range: 0.49 pg/ml - 120 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
Overview
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Product name
Porcine TGF alpha ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.6 pg/ml -
Range
0.49 pg/ml - 120 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 128.6 126% - 133% Plasma 122.5 88% - 142% Cell culture extracts 129.9 114% - 142% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Pig -
Product overview
Porcine TGF-alpha ELISA Kit (ab273225) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Porcine TGF-alpha in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Porcine TGF-alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Porcine TGF-alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Porcine TGF alpha coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Porcine TGF alpha Antibody 2 vials Porcine TGF alpha Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. -
Tissue specificity
Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. -
Sequence similarities
Contains 1 EGF-like domain. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
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Alternative names
- EGF like TGF
- EGF-like TGF
- ETGF
see all -
Database links
- Entrez Gene: 397484 Pig
- SwissProt: Q06922 Pig
Images
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Porcine TGF alpha ELISA Kit standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.