Pitstop® 2, Novel cell-permeable clathrin inhibitor (ab120687)
Key features and details
- Novel, selective cell-permeable clathrin inhibitor
- CAS Number: 1419093-54-1
- Purity: > 98%
Soluble in DMSO. Please refer to the Protocol Booklet for more information.
- Form / State: Solid
- Source: Synthetic
Overview
-
Product name
Pitstop® 2, Novel cell-permeable clathrin inhibitor -
Description
Novel, selective cell-permeable clathrin inhibitor -
Biological description
Novel, selective, cell membrane permeable clathrin inhibitor. Competitively inhibits clathrin terminal domain to selectively inhibit clathrin mediated endocytosis (CME) (IC50 = 12 μM for inhibition of amphiphysin association of clathrin TD). Interferes with receptor mediated endocytosis (RME), entry of HIV and synaptic vesicle recycling.
-
Purity
> 98% -
General notes
High concentrations of Pitstop 2™ may interfere with fluorescence imaging due to a low emittance of the compound in the green channel. This fluorescence is not usually detectable if the cells have been first fixed and washed prior to imaging.
Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Pitstop® is a trademark of Freie Universitat Berlin, Newcastle Innovation Ltd. and Children's Medical Research Institute.
-
CAS Number
1419093-54-1 -
Chemical structure
Properties
-
Chemical name
N-[5-(4-Bromobenzylidene)-4-oxo-4,5-dihydro-1,3-thiazol-2-yl]naphthalene-1-sulfonamide
-
Molecular weight
473.36 -
Molecular formula
C20H13BrN2O3S2 -
PubChem identifier
137254289 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO. Please refer to the Protocol Booklet for more information.
-
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
-
SMILES
C1=CC=C2C(=C1)C=CC=C2S(=O)(=O)N=C3NC(=O)/C(=C/C4=CC=C(C=C4)Br)/S3 -
Source
Synthetic
-
Research areas
Images
-
Functional Studies - Pitstop® 2, Novel cell-permeable clathrin inhibitor (ab120687) Dutta D et al. PLoS One. 2012; 7(9): e45799. doi: 10.1371/journal.pone.0045799 Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/
Hela cells were preincubated with DMSO (0.1%) or different doses of pitstop 2 (ab120687) ranging from 5 µM to 30 µM. Cells were then allowed to internalize Alexa594-Transferrin and antibodies to MHCI in the presence or absence of the drug for 30 min. After internalization, surface antibody was removed by low pH acid wash. Cells were then labeled with secondary antibodies to detect trasnferrin and MHCI.
Credit: Dutta D et al. PLoS One. 2012; 7(9): e45799. doi: 10.1371/journal.pone.0045799
-
Functional Studies - Pitstop® 2, Novel cell-permeable clathrin inhibitor (ab120687) Von Kleist L et al., Cell. 2011 Aug 5;146(3):471-84. Fig, 3(A-E)., doi: 10.1016/j.cell.2011.06.025.A) Pitstop® 2 reversibly inhibits Tf uptake. After 15 min preincubation HeLa cells were incubated with Alexa Fluor® 568-Tf in the presence of DMSO or 30 µM Pitstop 2 for 15 min. Tf uptake is seen to resume after washout of the drug for 1 hr. Scale bar, 10 mm. B) Reversibility and dose dependence of Pitstop® 2-mediated inhibition of Tf uptake. Data represent SEM (n = 3 independent experiments; *p -
-
-
-
-
-
-
-
-