Human TFF1 ELISA Kit
(ab277718)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.91 pg/ml
- Range: 12.5 pg/ml - 800 pg/ml
- Sample type: Cell culture media, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
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Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.5% Inter-assay Sample n Mean SD CV% Serum 3 3.3% -
Sample type
Urine, Serum, Cell culture media, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.91 pg/ml -
Range
12.5 pg/ml - 800 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 102 97% - 105% Serum 90 85% - 94% Cell culture media 88 87% - 89% Hep Plasma 80 80% - 90% EDTA Plasma 93 88% - 99% Cit plasma 97 95% - 99% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human TFF1 ELISA kit (ab277704) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human TFF1 protein in human serum, plasma, urine, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TFF1 with 2.91 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
TFF1 (Trefoil factor 1), also known as pS2, is a protein that in humans is encoded by the TFF1 gene. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. Trefoils are stable secretory proteins expressed in gastrointestinal mucosa. TFF1 function is not fully defined but is suggested to protect the gastric mucosa by stabilizing the mucus layer and affect healing of the epithelium. It is found that TFF1 in normal human urine inhibited the growth of calcium oxalate crystals. Urinary TFF1 showed an inhibitory potency similar to nephrocalcin. Concentrations and relative amounts of TFF1 in the urine of patients with idiopathic calcium oxalate kidney stones were significantly less than those found in controls. Mouse and rat share 67 % and 69 % sequence homology to human TFF1.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human TFF1 Capture Antibody 1 x 600µl 10X Human TFF1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human TFF1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine. -
Tissue specificity
Found in stomach, with highest levels in the upper gastric mucosal cells (at protein level). Detected in goblet cells of the small and large intestine and rectum, small submucosal glands in the esophagus, mucous acini of the sublingual gland, submucosal glands of the trachea, and epithelial cells lining the exocrine pancreatic ducts but not in the remainder of the pancreas (at protein level). Scattered expression is detected in the epithelial cells of the gallbladder and submucosal glands of the vagina, and weak expression is observed in the bronchial goblet cells of the pseudostratified epithelia in the respiratory system (at protein level). Detected in urine (at protein level). Strongly expressed in breast cancer but at low levels in normal mammary tissue. It is regulated by estrogen in MCF-7 cells. Strong expression found in normal gastric mucosa and in the regenerative tissues surrounding ulcerous lesions of gastrointestinal tract, but lower expression found in gastric cancer (at protein level). -
Sequence similarities
Contains 1 P-type (trefoil) domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- BCEI
- Breast cancer estrogen inducible protein
- Breast cancer estrogen inducible sequence
see all -
Database links
- Entrez Gene: 7031 Human
- Omim: 113710 Human
- SwissProt: P04155 Human
- Unigene: 162807 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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xample of human TFF1 standard curve in Sample Diluent NS.The TFF1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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. Interpolated concentrations of native TFF1 in human serum, plasma and urine samples.The concentrations of TFF1 were measured in duplicates, interpolated from the TFF1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2.5%, plasma (citrate) 6.25%, plasma (EDTA) 6.25%, plasma (heparin) 6.25%, and urine 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFF1 concentration was determined to be 18.85 ng/mL in serum, 2.85 ng/mL in plasma (citrate), 2.48 ng/mL in plasma (EDTA), 2.27 ng/mL in plasma (Heparin), and 152.70 ng/mL in urine.
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5% Serum from ten individual healthy human female donors was measured in duplicate. -
Recombinant Antibody BenefitsTo learn more about the advantages of recombinant antibodies see here.