Human GCSF Receptor ELISA Kit
(ab277707)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 9.45 pg/ml
- Range: 28 pg/ml - 1800 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
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Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 2.2% Inter-assay Sample n Mean SD CV% Extract 4 3.4% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
9.45 pg/ml -
Range
28 pg/ml - 1800 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 102 100% - 104% Serum 93 92% - 96% Cell Lysate 114 113% - 115% EDTA Plasma 92 92% - 94% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human GCSF Receptor ELISA kit (ab277707) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human GCSF Receptor in human serum, plasma (EDTA), cell culture supernatant, and cell and tissue extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human GCSF Receptor with 9.4 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Granulocyte colony-stimulating factor receptor (GCSF-R), also known as CD114, is a protein encoded by the CSF3R gene. GCSF Receptor belongs to a family of cytokine receptors known as the hematopoietin receptor family. GCSF Receptor acts as a receptor primarily for granulocyte colony-stimulating factor (CSF3), which is essential for granulocytic maturation. Binding of CSF3 to GCSF Receptor initiates a conformational change in the receptor, which leads to activation of several downstream pathways including JAK/STAT, PI3K/AKT, and MAPK/ERK. Mutations in CSF3R have been associated in most cases of severe congenital neutropenia. The antibodies used in this kit were raised against the extracellular portion of the canonical isoform of GCSF Receptor, also known as GCSFR-1.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human GCSF Receptor Capture Antibody 1 x 600µl 10X Human GCSF Receptor Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human GCSF Receptor Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface. -
Tissue specificity
One or several isoforms have been found in myelogenous leukemia cell line KG-1, leukemia U-937 cell line, in bone marrow cells, placenta, and peripheral blood granulocytes. Isoform GCSFR-2 is found only in leukemia U-937 cells. Isoform GCSFR-3 is highly expressed in placenta. -
Involvement in disease
Hereditary neutrophilia -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- CD 114
- CD114
- CD114 antigen
see all -
Database links
- Entrez Gene: 1441 Human
- Omim: 138971 Human
- SwissProt: Q99062 Human
- Unigene: 524517 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human GCSF Receptor standard curve in Sample Diluent NS.The GCSF Receptor standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human GCSF Receptor standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.The GCSF Receptor standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native GCSF Receptor in THP-1 cell extract. Undiluted samples are as follows: THP-1 cell extract, 1,000 µg/ml.The concentrations of GCSF Receptor were measured in duplicate and interpolated from the GCSF Receptor standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Serum from eight individual healthy GCSF Receptor gender donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GCSF Receptor concentration was determined to be 368 pg/mL with a range of 58 – 1,119 pg/mL.
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Recombinant Antibody BenefitsTo learn more about the advantages of recombinant antibodies see here.