Phosphoglycerate Kinase Activity Assay Kit (Colorimetric) (ab252890)
Key features and details
- Detection method: Colorimetric
- Platform: Microplate (12 x 8 well strips)
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 50 mU/well
Overview
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Product name
Phosphoglycerate Kinase Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Tissue, Adherent cells, Suspension cells -
Sensitivity
50 mU/well -
Assay duration
Multiple steps standard assay -
Product overview
Phosphoglycerate Kinase Activity Assay Kit (Colorimetric) ab252890 provides a quick and easy way for monitoring PGK activity in various samples. In the first step of this enzymatic assay, PGK converts 3-Phosphoglycerate and ATP to 1,3-Bisphosphoglycerate and ADP. The nascent intermediate is detected via a series of enzymatic reactions that lead the oxidation of NADH to NAD , which can be easily detected (OD= 340 nm). The assay is simple, sensitive and can detect Phosphoglycerate Kinase Activity lower than 50 mU in variety of samples.
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Tested applications
Suitable for: Functional Studiesmore details -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests ATP 2 vials NADH 1 vial PGK Developer 1 vial PGK Positive Control 1 vial PGK Substrate 1 vial PGK Assay Buffer 1 x 25ml U.V. Transparent Plate (96-well) 1 unit -
Research areas
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Function
In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. -
Involvement in disease
Defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations. -
Sequence similarities
Belongs to the phosphoglycerate kinase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- Cell migration-inducing gene 10 protein
- Epididymis secretory sperm binding protein Li 68p
- HEL S 68p
see all
Images
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Example Data
Relative PGK Activity was calculated in lysates prepared from rat liver (2 µg), rat muscle (0.5 µg), HeLa (Human epithelial cell line from cervix adenocarcinoma) (1.5 µg) and Jurkat (Human T cell leukemia cell line from peripheral blood) (1.5 µg).
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Example DataKinetic measurement of Phosphoglycerate Kinase activity from various samples.
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Example DataNADH standard curve.
