Phosphatidic Acid Assay Kit (Fluorometric) (ab273335)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Cell culture extracts, Tissue Extracts
Overview
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Product name
Phosphatidic Acid Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Quantitative -
Assay duration
Multiple steps standard assay -
Product overview
Phosphatidic Acid Assay Kit (Fluorometric) (ab273335) is a plate-based enzymatic assay for quantitation of Phosphatidic Acid (PA) in cells and tissues.
The PA Converter hydrolyzes PA to form an intermediate which, in the presence of a developer and enzyme mix, converts a non-fluorescent probe to a fluorescent product (Ex/Em= 535/587 nm) that can be quantified. The Converter is specific to PA and does not hydrolyze other phospholipids (e.g. those with more complex head groups such as phosphatidylcholine or cardiolipin), allowing direct quantitation of PA.
This kit can detect as low as 40 pmol PA per well. -
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests PA Assay Buffer 1 x 100ml PA Converter Lyophilized 1 vial PA Developer Lyophilized 1 vial PA Enzyme Mix Lyophilized 1 vial PA Standard (1 mM) 1 x 100µl Probe in DMSO 1 x 400µl
Images
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Example data
Example data.
Phosphatidic acid standard curve.
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Example dataExample data.
Phosphatidic acid content in rat liver and HEK 293 cells. For rat liver extract, 100 mg tissue was homogenized in 1 ml PA assay buffer. Lipid extraction was carried out and was re-suspended in 200 µl 5% Triton X-100 solution. For HEK293, cells were grown to confluency and harvested using a rubber policeman.
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Example dataExample data.
Assay Specificity: Equimolar amounts (20 nmol) of phosphatidic acid (PA), phosphatidylcholine (PC), phosphatidylinositol (PI) and cardiolipin (CL) show interference less than 20% with respect of Phosphatidic Acid. Assay shows specificity for phosphatidic acid.