Phenolic Compounds Assay Kit (Colorimetric) (ab273293)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
- Sensitivity: 0.02 mM
Overview
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Product name
Phenolic Compounds Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Assay type
Quantitative -
Sensitivity
0.02 mM -
Assay duration
Multiple steps standard assay -
Product overview
Phenolic Compounds Assay Kit (Colorimetric) (ab273293) provides a quick, sensitive and selective method for measuring the total amount of phenolic compounds in various biological samples. In this assay, phenolic compounds couple with diazonium salts under alkaline conditions to form a stable diazo chromophore, detectable by absorbance at 480 nm. Unlike the classical Folin-Ciocalteu (FC) protocol for measuring phenolic compounds, our assay is not affected by non-phenolic reducing substances such as sulfites, reducing sugars or ascorbic acid. The assay is high-throughput adaptable and can detect concentrations of phenolic compounds as low as 0.02 mM catechin equivalents (CEs) from a variety of plant and food-based samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests Catechin Standard (100 mM) 1 x 100µl PC Assay Buffer 1 x 25ml PC Probe 1 x 4ml Vanillic Acid (50 mM) 1 x 500µl
Images
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Catechin standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Absorbance readings for green tea, blueberries, orange juice and vanillic acid positive control.Absorbance readings for 50 μL diluted solutions of green tea (brewed for 5 minutes and diluted 1:20 fold with ddH2O), blueberry methanolic extract (extract made from 50 mg of freeze-dried blueberries in 5 mL of MeOH/ddH2O/HCl extraction solvent and diluted 1:5 fold with ddH2O), orange juice (centrifuged to remove pulp and supernatant used without dilution) and 50 μL positive control (vanillic acid 50 mM solution).
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Catechin equivalents (in mM) of green tea, blueberries and orange juice.Catechin equivalency is defined as nmoles of phenolic compounds per µl of solution, equivalent to nmoles of catechin per µl of solution, as calculated from the Catechin Standard curve. Assays were performed following the kit protocol.