Phalloidin-iFluor 514 Reagent (ab176754)
Key features and details
- Assay type: Cell-based (qualitative)
- Platform: Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Phalloidin-iFluor 514 Reagent
See all F-actin kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (qualitative) -
Product overview
Phalloidin-iFluor 514 Reagent (ab176754) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phalloidin-iFluor 514 can be easily detected with a fluorescent microscope at Ex/Em = 520/547 nm.
Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.
Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the dissociation rate of actin subunits from filaments ends, essentially stabilizing actin filaments through the prevention of filament depolymerization.
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Notes
Actin is a globular, roughly 42-kDa protein found in almost all eukaryotic cells. It is also one of the most highly conserved proteins, differing by no more than 20% in species as diverse as algae and humans. Actin is the monomeric subunit of two types of filaments in cells: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells. Thus, actin participates in many important cellular processes including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, as well as the establishment and maintenance of cell junctions and cell shape.
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Platform
Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 300 tests Phalloidin-iFluor 514 Conjugate 1 x 300 tests -
Research areas
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Alternative names
- actin filament
- f actin
- Filamentous actin
Images
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CytoPainter Phalloidin-iFluor 514 Reagent (ab176754)
Excitation and emission spectra of phalloidin-iFluor 514 reagent.