PGF2 alpha High Sensitivity ELISA Kit (ab133056)
Key features and details
- Sensitivity: 0.98 pg/ml
- Range: 1.95 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
Overview
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Product name
PGF2 alpha High Sensitivity ELISA Kit
See all PGF2 alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 107.5pmol/ml 24 6.53% 33.79pmol/ml 24 4.94% 11.5pmol/ml 24 7.16% Inter-assay Sample n Mean SD CV% 117.8pmol/ml 10.97% 35.81pmol/ml 8.88% 10.1pmol/ml 10.14% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma -
Assay type
Competitive -
Sensitivity
0.98 pg/ml -
Range
1.95 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Saliva 103.5 % - % Urine 107 % - % Serum 100.3 % - % Plasma 96.9 % - % -
Assay time
2h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Predicted to work with: a wide range of other species
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Product overview
Abcam’s PGF2α High Sensitivity in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of PGF2α in milk, culture supernatants, saliva, urine, serum and plasma.
A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-PGF2α antigen and a polyclonal sheep antibody specific to PGF2α. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of PGF2α captured in the plate.
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Notes
Prostaglandin F2α (PGF2α) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF2α is often viewed as an antagonist to PGE2 due to their opposing effects on various tissues. PGF2α is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF2α is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF2α have also been associated with preeclampsia.
Compound Cross Reactivity PGF2α 100% PGF1α 11.82% PGD2 3.62% 6-keto-PGF1α 1.38% PGI2 1.25% PGE2 0.77% Thromboxane B2 0.77% 8-iso PGF2α 0.73% PGE1 0.39% PGA2 6, 15-keto-13, 14-dihydro-PGF1α 2-Arachidonoylglycerol Anandamide -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Assay Buffer 1 x 27ml Donkey anti-sheep IgG Microplate (12 x 8 wells) 1 unit PGF2α Alkaline Phosphatase Conjugate 1 x 2.5ml PGF2α Antibody 1 x 2.5ml PGF2α Standard 1 x 0.5ml Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml -
Research areas
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Alternative names
- Prostaglandin F2 alpha
Images
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Typical Standard Curve
Representative Standard Curve using ab133056.
