Peroxide Assay Kit (ab272537)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Urine
Overview
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Product name
Peroxide Assay Kit
See all Hydrogen Peroxide kits -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media -
Assay type
Quantitative -
Range
0.2 µM - 30 µM -
Product overview
Peroxide Assay Kit (ab272537) is a simple, direct and automation-ready procedures for quantitative determination of peroxide find wide applications in research and drug discovery. This assay kit is designed to measure peroxide concentration in biological samples without any pretreatment. The improved method utilizes the chromogenic Fe3+-xylenol orange reaction, in which a purple complex is formed when Fe2+ provided in the reagent is oxidized to Fe3+ by peroxides present in the sample. The intensity of the color, measured at 540-610nm, is an accurate measure of the peroxide level in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
Sensitive and accurate. Enhanced color intensity using sorbitol. Detection range 0.2 µM (7 ng/mL) to 30 µM (1,020 ng/mL) H2O2 in 96-well plate assay.
Simple and high-throughput. The procedure involves addition of a single detection reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.
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Tested applications
Suitable for: Functional Studiesmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 250 tests Reagent A 1 x 1ml Reagent B 1 x 50ml Standard (3% stabilized H2O2) 1 x 100µl -
Research areas
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Relevance
Hydrogen Peroxide (H2O2) is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-kB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down’s syndrome and immune system diseases. -
Alternative names
- H2O2
Images
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Peroxide Assay Kit Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.