PE Anti-CD68 antibody [FA-11] (ab216701)
![PE Anti-CD68 antibody [FA-11] (ab216701)](https://www.abcam.com/ps/products/216/ab216701/Images/ab216701-292259-anti-cd68-antibody-fa-11-phycoerythrin-flow-cytometry.jpg "PE Anti-CD68 antibody [FA-11] (ab216701)")
Key features and details
- PE Rat monoclonal [FA-11] to CD68
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Overview
-
Product name
PE Anti-CD68 antibody [FA-11]
See all CD68 primary antibodies -
Description
PE Rat monoclonal [FA-11] to CD68 -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
-
Immunogen
Tissue, cells or virus corresponding to Mouse CD68.
-
Positive control
- Flow Cyt: Raw264.7 cells
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
FA-11 -
Isotype
IgG2a -
Research areas
Images
-
Flow Cytometry - PE Anti-CD68 antibody [FA-11] (ab216701)
Overlay histogram showing Raw264.7 cells stained with ab216701 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab216701, 1/1000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in Raw264.7 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
