PAF Acetylhydrolase Assay Kit (ab133088)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell Lysate, Plasma, Serum, Tissue Homogenate
Overview
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Product name
PAF Acetylhydrolase Assay Kit
See all PAF Acetylhydrolase kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% PAF-AH 89 3.5% Inter-assay Sample n Mean SD CV% PAF-AH 89 10% -
Sample type
Serum, Plasma, Cell Lysate, Tissue Homogenate -
Assay type
Enzyme activity -
Range
0.02 µmol/min/ml - 0.2 µmol/min/ml -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Abcam's PAF Acetylhydrolase Assay Kit (ab133088) provides an accurate and convenient method for measurement of PAF acetylhydrolase (PAF-AH) activity (both cytosolic and extracellular). The assay uses 2-thio PAF which serves as a substrate for all PAF-AHs. Upon hydrolysis of the acetyl thioester bond at the sn-2 position by PAF-AH, free thiols are detected using 5,5’-dithio-bis-(2- nitrobenzoic acid).
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Notes
Platelet-activating factor (PAF) is a biologically active phospholipid synthesized by a variety of cells upon stimulation. PAF is converted to the biologically inactive lyso-PAF by the enzyme PAF acetylhydrolase (PAF-AH). PAF-AHs are located intra- and extra-cellularly (for instance, in both cytosol and plasma) Plasma PAF-AH is highly selective for phospholipids with very short acyl groups at the sn-2 position and is associated with lipoproteins.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 96 tests 2-thio PAF (substrate) 2 vials 96-Well Plate Cover 1 unit 96-Well Solid Plate (Colorimetric Assay) 1 unit DTNB 4 vials Human Plasma PAF-AH Standard 1 vial PAF Acetylhydrolase Assay Buffer 1 1 vial PAF Acetylhydrolase Assay Buffer 2 1 vial -
Research areas
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Function
Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities. -
Sequence similarities
In the N-terminal section; belongs to the asparaginase 1 family.
Contains 5 ANK repeats. - Information by UniProt
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Alternative names
- 60 kDa lysophospholipase
- A530050D06Rik
- AI429460
see all
Images
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Activity of Human PAF-AH
Representative Standard Curve using ab133088.
