Optiblot SDS-PAGE Sample Preparation Kit (ab133414)
Overview
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Product name
Optiblot SDS-PAGE Sample Preparation Kit -
Product overview
The Optiblot SDS-PAGE Sample Preparation Kit (ab133414) provides a means to quickly concentrate protein samples, and separate them from buffers that interfere with electrophoresis.
The fast, efficient protocol generates samples ready to load on a gel in less than ten minutes, much more quickly than can be achieved with alternate methods such as dialysis, or acetone or TCA precipitation. Additionally, the Optiblot SDS-PAGE Sample Preparation protocol is easily scaled up, allowing multiple samples to be prepared in parallel.
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Tested applications
Suitable for: Purificationmore details
Properties
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Components 10 tests 25 tests Extraction loading buffer 1 x 1ml 1 x 1ml Protein filters 10 units 25 units Resin 1 x 500ml 2 x 500ml -
Research areas
Images
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SDS-PAGE - Optiblot SDS-PAGE Sample Preparation Kit (ab133414)Resin concentration is compatible with Western blot detection. Western blots were created with samples of A431 cell extract (panels a and b, lane 2), diluted cell extract (lane 3) and the diluted extract concentrated using the Optiblot SDS-PAGE Sample Preparation Kit protocol (lane 4). The blots were detected using the Optiblot Fluorescent Western Blot Kit (ab133410). No bands were detectable in the diluted cell extract when stained for either GAPDH (panel a, lane 3) or SRC (panel b, lane 3). However, after resin concentration, bands are easily visualized for both proteins (lanes 4). Band intensities are indicated.
