Nitrotetrazolium blue chloride (NBT), Chromogenic substrate (ab146262)
Key features and details
- Chromogenic substrate.
- CAS Number: 298-83-9
- Soluble in water to 10 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Nitrotetrazolium blue chloride (NBT), Chromogenic substrate -
Description
Chromogenic substrate. -
Alternative names
- Nitro BT
- Nitro-Blue tetrazolium chloride
- NSC 27622
see all -
Biological description
Chromogenic substrate for dehydrogenases and other oxidases that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. Acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase. Used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP).
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CAS Number
298-83-9 -
Chemical structure
Properties
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Chemical name
3,3'-[3,3'-Dimethoxy(1,1'-biphenyl)-4,4'-diyl]bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium dichloride -
Molecular weight
817.64 -
Molecular formula
C40H30Cl2N10O6 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 10 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic
