NIH/3T3 whole cell lysate (TPA stimulated) (ab14878)
Overview
-
Product name
NIH/3T3 whole cell lysate (TPA stimulated)
See all NIH 3T3 lysates -
Description
NIH/3T3 whole cell lysate -
General notes
Cell line: NIH/3T3 (Mouse embryonic fibroblast).
Cells were cultured in medium supplemented with 50 ng/ml TPA (phorbol, 12-myristate, 13 acetate) for 2 hours at 37°C immediately prior to harvesting.
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Properties
-
Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 6.80
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol -
Concentration information loading... -
Research areas
-
Background
NIH 3T3 cells are established from a NIH Swiss mouse embryo. These cells are highly contact inhibited and are sensitive to sarcoma virus focus formation and leukaemia virus propagation. Cells have now lost their contact inhibition. This cell line was established from NIH Swiss mouse embryo cultures in the same manner as the original random bred 3T3 and the inbred BALB/c 3T3. The established NIH/3T3 line was subjected to more than 5 serial cycles of subcloning in order to develop a subclone with morphologic characteristics best suited for transformation assays. It is therefore used for DNA transfection studies
