Nifuroxazide, JAK/STAT signaling inhibitor (ab120951)
Key features and details
- JAK/STAT signaling inhibitor. Nitrofuran antibiotic.
- CAS Number: 965-52-6
- Purity: > 98%
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Nifuroxazide, JAK/STAT signaling inhibitor -
Description
JAK/STAT signaling inhibitor. Nitrofuran antibiotic. -
Biological description
JAK/STAT signaling inhibitor. Nitrofuran antibiotic. Inhibits JAK2 and Tyk2 phosphorylation and subsequent downstream STAT3 inhibition (EC50 = 3 μM). Antidiarrheal. Shows little effect on Akt, NF-κB, JAK1, MAPK and Src signaling.
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Purity
> 98% -
CAS Number
965-52-6 -
Chemical structure
Properties
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Chemical name
4-Hydroxybenzoic acid 2-[(5-nitro-2-furanyl)methylene]hydrazide -
Molecular weight
275.22 -
Molecular formula
C12H9N3O5 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic
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Research areas
Images
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Functional Studies - Nifuroxazide, JAK/STAT signaling inhibitor (ab120951)
FOX-NY cells were incubated at 37°C for 30 minutes with vehicle control (0 µM) and different concentrations of nifuroxazide (ab120951). Decreased expression of Stat3 (phospho Y705) (ab76315) in FOX-NY cells correlates with an increase in nifuroxazide concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab76315 at 1/20000 dilution and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.